Mice homozygous for this Ifnar1 knock-out mutation are more susceptible to viral infection, have an abnormal NK antitumor response, and reduced bone mass. This mutant mouse strain may be useful in studies of immunological responses to infection, and bone homeostasis. Mouse mutants involving this gene have been used in studies of Zika virus pathogenesis. Note: these mice carry the same allele as 'A129' mice, although on a congenic C57BL/6, not 129, genetic background.
Dr. Michel Aguet, EPFL, Ecole polytechnique fédérale de Lausanne
Genetic Background | Generation |
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N6+N1F8
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ifnar1 | interferon (alpha and beta) receptor 1 |
Mice that are homozygous for the targeted mutation are viable, fertile, and normal in size. No gene product (mRNA) is detected by Northern blot analysis of homozygous primary embryonic fibroblasts. Mice that are homozygous for this targeted allele lack type I IFN receptor function, exhibit enhanced osteoclastogenesis with decreased bone density, and impaired response to protozoan parasite (Leishmania) infection. NK cell mediated tumor regression does not occur in homozygotes in response to B16 melanoma tumor implant model. Mouse mutants involving this gene have been used in studies of Zika virus pathogenesis.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 3. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The mice were backcrossed to C57Bl/6 for at least 5 generations, as reported by the donating investigator.
Allele Name | targeted mutation 1, Michel Aguet |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | A 129; A-; A129; IFN Alpha Ro/o; IFN type I receptor; IFN-alpha/betaR-; IFN-alpha/betaR/A129; IFN-alpha/betaR0; IFN-alphabetaR-; IFN-alphabeta-R KO; IFNAR(0); IFNAR-; Ifnar1-; IFN-IR KO; IFN-IR-; IFNR-; IFNRA1,-.; IFNRI -; IFNRI KO |
Gene Symbol and Name | Ifnar1, interferon (alpha and beta) receptor 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 16 |
Molecular Note | A neomycin cassette was inserted into exon 3 at a position corresponding to amino acid 88. |
Mutations Made By | Dr. Michel Aguet, EPFL, Ecole polytechnique fédérale de Lausanne |
When maintaining a live colony, these mice can be bred as homozygotes. SPF conditions are recommended.
When using the IFN-αβR- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32045 in your Materials and Methods section.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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