B6;C-GhrhrlitPrkdcscid/BmJ mice are deficient in growth hormone and IGF1 and are useful for determining endocrine dependence of grafted cells and tissues (Beamer et al., 1993, Cancer Research, v53:3741; Friend et al., 2001 Growth Hormone & IGF Research, v11:84).
A homozygote from the strain C57BL/6J-Ghrhrlit (stock #000533) was bred with a C.BKa-Ighb/Icr-Prkdsscid homozygote and the resulting offspring were intercrossed to generate a strain homozygous for Prkdcscid and segregating for Ghrhrlit. This strain was generated and maintained by sibling inbreeding in the laboratory of Dr. Wesley Beamer. In 2009 sperm were cryopreserved from males at generatiuon F31 that were homozygous for both the little and scid mutations. Cryorecovery using eggs from CBySmn.CB17-Prkdc
|Allele Synonym(s)||lit; litm|
|Gene Symbol and Name||Ghrhr, growth hormone releasing hormone receptor|
|Gene Synonym(s)||GHRFR; GHRHREC; GRFR; Ghrfr; Ghrfr; IGHD1B; growth hormone releasing factor receptor; lit; little|
|Strain of Origin||C57BL/6J|
|Molecular Note||The mutation in little mice is a A-to-G transition in codon 60 that is predicted to alter this residue from a aspartate to a glycine in the encoded protein. Reduced levels of mRNA were noted in total RNA derived from pituitary of homozygous mice, and invitro assays demonstrated that no functional protein is made from this allele.|
|Allele Name||severe combined immunodeficiency|
|Allele Synonym(s)||SCID; scid|
|Gene Symbol and Name||Prkdc, protein kinase, DNA activated, catalytic polypeptide|
|Gene Synonym(s)||AI326420; AU019811; DNA-PK; DNA-PKcs; DNAPDcs; DNAPK; DNPK1; DOXNPH; HYRC; HYRC1; IMD26; XRCC7; doxorubicin nephropathy; dxnph; dxnph; expressed sequence AI326420; expressed sequence AU019811; p350; scid; severe combined immunodeficiency; slip|
|Strain of Origin||C.B-17|
|Molecular Note||A T-to-A transversion point mutation at a position corresponding to codon 4095 created a premature stop codon.|
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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