These double mutant mice are deficient in both growth hormone and IGF1.
Wes Beamer, JAX
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Ghrhr | growth hormone releasing hormone receptor |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Prkdc | protein kinase, DNA activated, catalytic polypeptide |
B6;C-GhrhrlitPrkdcscid/BmJ mice are deficient in growth hormone and IGF1 and are useful for determining endocrine dependence of grafted cells and tissues (Beamer et al., 1993, Cancer Research, v53:3741; Friend et al., 2001 Growth Hormone & IGF Research, v11:84).
A homozygote from the strain C57BL/6J-Ghrhrlit (stock #000533) was bred with a C.BKa-Ighb/Icr-Prkdsscid homozygote and the resulting offspring were intercrossed to generate a strain homozygous for Prkdcscid and segregating for Ghrhrlit. This strain was generated and maintained by sibling inbreeding in the laboratory of Dr. Wesley Beamer. In 2009 sperm were cryopreserved from males at generatiuon F31 that were homozygous for both the little and scid mutations. Cryorecovery using eggs from CBySmn.CB17-Prkdc
Allele Name | little |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | lit; litm |
Gene Symbol and Name | Ghrhr, growth hormone releasing hormone receptor |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 6 |
Molecular Note | The mutation in little mice is a A-to-G transition in codon 60 that alters this residue from a aspartate to a glycine in the encoded protein (p.D60G). Reduced levels of mRNA were noted in total RNA derived from pituitary of homozygous mice, and in vitro assays demonstrated that no functional protein is made from this allele. |
Allele Name | severe combined immunodeficiency |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | SCID |
Gene Symbol and Name | Prkdc, protein kinase, DNA activated, catalytic polypeptide |
Gene Synonym(s) | |
Site of Expression | T and B lymphocytes. |
Strain of Origin | C.BKa-Ighb/Icr |
Chromosome | 16 |
Molecular Note | A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*). |
When using the lit/scid mouse strain in a publication, please cite the originating article(s) and include JAX stock #010816 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Prkdc<scid>, Heterozygous for Ghrhr<lit> |
Frozen Mouse Embryo | B6;C-Ghrhr<lit> Prkdc<scid>/BmJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;C-Ghrhr<lit> Prkdc<scid>/BmJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;C-Ghrhr<lit> Prkdc<scid>/BmJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;C-Ghrhr<lit> Prkdc<scid>/BmJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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