STOCK Tg(mI56i-flpe)39Fsh/J

Stock number: 010815
Product name: Dlx5/6-Flpe
Research areas: Research Tools

Description

These Dlx5/6-Flpe mice have expression of Myc-tagged FLPe recombinase directed to GABAergic neuron populations in the forebrain. When bred with mice containing an FRT-flanked sequence, FLPe-mediated recombination will result in deletion of the FRT-flanked sequence in forebrain GABAergic populations of the offspring.

Detailed description

Hemizygous Dlx5/6-Flpe mice are viable and fertile, with expression of Myc-tagged FLPe recombinase directed to GABAergic neuron populations in the forebrain by the mouse Dlx5/Dlx6 (id6/id5) intergenic enhancer region and β-globin basal promoter. The donating investigator reports that no abnormal phenotype is observed in hemizygous mice. When bred with mice containing an FRT-flanked sequence, FLPe-mediated recombination will result in deletion of the FRT-flanked sequence in forebrain GABAergic populations of the offspring.

Development

The Dlx5/6-Flpe transgene was designed with the intergenic enhancer region between the mouse Dlx5 and Dlx6 (id6/id5) locus together with a β-globin basal promoter, a chimeric intron containing a human β-globin splice donator site and IgHC variable region splice acceptor site, a Flpe site-specific recombinase with Myc tag (FlpeMyc) sequence, and SV40 early mRNA polyadenylation signal cassette. This transgene was microinjected into FVB/N mouse cells. Mice from founder line 39 were mated with outbred Swiss Webster mice for many generations. Dlx5/6-Flpe mice predominantly on an outbred Swiss Webster genetic background (and homozygous for the the retinal degeneration allele Pde6b^rd1) were sent to The Jackson Laboratory. Upon arrival, sperm from transgenic mice was cryopreserved. Some of the cryopreserved sperm was used to fertilize oocytes from FVB/NJ inbred mice (Stock No. 001800) to establish the Dlx5/6-Flpe colony. When maintained by breeding Dlx5/6-Flpe mice together or to FVB/NJ inbred mice, the Dlx5/6-Flpe mice should also be homozygous for the retinal degeneration allele Pde6b^rd1.

Allele

Symbol: Tg(mI56i-flpe)39Fsh
Name: transgene insertion 39, Gordon Fishell
MGI accession ID: MGI:4421157
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(mI56i-flpe)39Fsh
Marker name: transgene insertion 39, Gordon Fishell
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: FLP,FLP recombinase,Saccharomyces cerevisiae; myc tag,myc tag,
Site of expression: Myc-tagged FLPe recombinase is expressed in GABAergic neuron populations in the forebrain.
Molecular note: The Dlx5/6-FLPe transgene was designed with the intergenic enhancer region between the mouse Dlx5 and Dlx6 (id6/id5, mI56i) locus together with a beta-globin basal promoter, a chimeric intron containing a human _-globin splice donator site and IgHC variable region splice acceptor site, a FLPe site-specific recombinase with Myc tag (FLPeMyc) sequence, and SV40 early mRNA polyadenylation signal cassette. This transgene was microinjected into FVB/N mouse cells. Mice from founder line 39 were mated with outbred Swiss Webster mice for many generations.