NOD/ShiLtJ-mt^FVB/NJ/IbraJ

Stock number: 010809
Product name: NOD-mt^FVB
Research areas: Diabetes and Obesity Research, Research Tools

Description

This conplastic strain, with a mutation in the mitochondrial mt-Atp8 gene, exhibits a higher diabetes incidence and may be useful for studies related to type I diabetes and as a tool for dissection of quantitative trait loci.

Detailed description

This strain is one of several conplastic strains created to investigate mitochondrial DNA variations on complex traits. The FVB/NJ strain carries an aspartic acid to tyrosine substitution in the mitochondrial mt-Atp8 gene. On the NOD/ShiLtJ background, this conplastic strain exhibits a higher incidence of diabetes.

Development

Dr. Saleh Ibrahim from the University of Lubeck developed the NOD/ShiLtJ-mt^FVB/NJ/IbraJ conplastic strain by sequential backcrossing of the FVB/NJ donor female to the NOD/ShiLtJ host male. A conplastic strain carries the mitochondrial genome of the donor strain. The set was donated to The Jackson Laboratory in 2009.

Allele

Symbol: mt-Atp8^m1
Name: mutation 1
MGI accession ID: MGI:4355536
Generation method: Spontaneous
Marker symbol: mt-Atp8
Marker name: mitochondrially encoded ATP synthase 8
Chromosome: MT
Strain of origin: FVB/NJ
Molecular note: A G-to-T transversion is located at nucleotide 7778 resulting in an aspartic acid to tyrosine substitution in the fifth amino acid of the conserved N-terminus of the protein (p.D5Y). This substitution has been reported in the FVB/NJ strain.