This conplastic strain, with a mutation in the mitochondrial mt-Atp8 gene, exhibits a higher diabetes incidence and may be useful for studies related to type I diabetes and as a tool for dissection of quantitative trait loci.
Saleh Ibrahim, University of Luebeck
Genetic Background | Generation |
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001976 NOD/ShiLtJ |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | mt-Atp8 | mitochondrially encoded ATP synthase 8 |
This strain is one of several conplastic strains created to investigate mitochondrial DNA variations on complex traits. The FVB/NJ strain carries an aspartic acid to tyrosine substitution in the mitochondrial mt-Atp8 gene. On the NOD/ShiLtJ background, this conplastic strain exhibits a higher incidence of diabetes.
Dr. Saleh Ibrahim from the University of Lubeck developed the NOD/ShiLtJ-mtFVB/NJ/IbraJ conplastic strain by sequential backcrossing of the FVB/NJ donor female to the NOD/ShiLtJ host male. A conplastic strain carries the mitochondrial genome of the donor strain. The set was donated to The Jackson Laboratory in 2009.
Allele Name | mutation 1 |
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Allele Type | Spontaneous |
Allele Synonym(s) | mt-Atp8FVB/NJ |
Gene Symbol and Name | mt-Atp8, mitochondrially encoded ATP synthase 8 |
Gene Synonym(s) | |
Strain of Origin | FVB/NJ |
Chromosome | MT |
Molecular Note | A G-to-T transversion is located at nucleotide 7778 resulting in an aspartic acid to tyrosine substitution in the fifth amino acid of the conserved N-terminus of the protein (p.D5Y). This substitution has been reported in the FVB/NJ strain. |
While maintaining a live colony, these mice are mated brother x sister or carrier females mated to NOD/ShiLtJ males.
When using the NOD-mtFVB mouse strain in a publication, please cite the originating article(s) and include JAX stock #010809 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for mt<FVB/NJ>, 1 pair minimum |
Frozen Mouse Embryo | NOD/ShiLtJ-mt<FVB/NJ>/IbraJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD/ShiLtJ-mt<FVB/NJ>/IbraJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD/ShiLtJ-mt<FVB/NJ>/IbraJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | NOD/ShiLtJ-mt<FVB/NJ>/IbraJ Frozen Embryos | $3373.50 |
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