B6.129P2(Cg)-Hprt^{tm41(Ple160-lacZ)Ems}/Mmjax

Stock number: 010805
Strain synonyms: B6.129P2(Cg)-Hprt
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple160-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human PITX3 gene direct expression of β-galactosidase.

Allele

Symbol: Hprt^{tm41(Ple160-lacZ)Ems}
Name: targeted mutation 41, Elizabeth M Simpson
MGI accession ID: MGI:4834790
Generation method: Targeted
Marker symbol: Hprt
Marker name: hypoxanthine guanine phosphoribosyl transferase
Chromosome: X
Strain of origin: (B6.129P2-Hprt^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in the anterior thalamus, with further expression observed in the ventral-lateral hippocampus, and the VTA region.
Molecular note: The Ple160-lacZ transgene (pEMS1631) was designed with the 2538 bp Ple160 minipromoter (PITX3-B; derived from a subsection of the promoter from the human paired-like homeodomain 3 (PITX3) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

The promoter/regulatory regions of the human paired-like homeodomain 3 (PITX3) gene directs expression of lacZ to anterior thalamus, with further expression observed in the ventral-lateral hippocampus, and the VTA region. The latter (VTA region) location reflects the expression of the endogenous Pitx3 gene.
General note: Germ line transmission of mutant cell line mEMS4135 has been established.