| Allele Name | targeted mutation 41, Elizabeth M Simpson |
| Allele Type | Targeted |
| Allele Synonym(s) | Hprttm41(mEMS4135)Ems; PITX3-B-lacZ; Ple160 |
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
| Gene Synonym(s) | |
| Promoter | PITX3, paired like homeodomain 3, human |
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| Expressed Gene | lacZ, beta-galactosidase, E. coli |
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| Site of Expression | lacZ is expressed in the anterior thalamus, with further expression observed in the ventral-lateral hippocampus, and the VTA region. |
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| Strain of Origin | (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
| Chromosome | X |
| General Note | Germ line transmission of mutant cell line mEMS4135 has been established. |
| Molecular Note | The Ple160-lacZ transgene (pEMS1631) was designed with the 2538 bp Ple160 minipromoter (PITX3-B; derived from a subsection of the promoter from the human paired-like homeodomain 3 (PITX3) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human paired-like homeodomain 3 (PITX3) gene directs expression of lacZ to anterior thalamus, with further expression observed in the ventral-lateral hippocampus, and the VTA region. The latter (VTA region) location reflects the expression of the endogenous Pitx3 gene. |
| Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
