Overview

Also Known As: Gad2-IRES-Cre

Gad2-IRES-Cre knock-in mice have Cre recombinase expression directed to GAD2 positive neurons. These mice may be used to generate conditional mutations for studying GAD2 cell populations.
Of note, the same Gad2-IRES-Cre knock-in allele is also available as C57BL/6N-congenic (Stock No. 019022) and C57BL/6J-congenic (Stock No. 028867).

Donating Investigator

Z. Josh Huang, Cold Spring Harbor Laboratory

Genetic overview

Genetic Background	Generation
	F?+N1F6 (2019-03-18 00:00:00)

Gad2^tm2(cre)Zjh

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Gad2	glutamic acid decarboxylase 2

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$333.00 Domestic price for female 4-week

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Details

Detailed Description

The Gad2-IRES-Cre knock-in allele has an internal ribosome entry site and Cre recombinase in the 3' UTR of the glutamic acid decarboxylase 2 locus (Gad2). As such, the endogenous Gad2 promoter/enhancer elements direct cre expression to GAD2 positive neurons. When Gad2-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Gad2-expressing cells (GAD2 positive neurons) of the offspring. Gad2 expression from the Gad2-IRES-Cre allele has not been evaluated. Additional phenotype information described below.

In 2010, the donating investigator reported Cre recombinase activity is specific and efficient (largely recapitulates the endogenous Gad2 expression pattern with highly efficient recombination). The donating investigator did not examine cre expression in tissues other than brain. Gad2 expression from the Gad2-IRES-Cre allele was not evaluated. They also report that homozygous mice are viable and fertile.

For characterization information of the Gad2-IRES-Cre knock-in allele, see images at the Allen Institute for Brain Science website (Gad2-IRES-Cre images).

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Gad2^tm2(cre)Zjh). This same information would also be found searching the MGI Recombinase Activity database.

Development

The Gad2-IRES-Cre knock-in allele was designed by Dr. Z. Josh Huang (Cold Spring Harbor Laboratory). A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the glutamic acid decarboxylase 2 locus (Gad2) on chromosome 2. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10); see Stock No. 005703) to generate the colony and remove the neo selection cassette. These Gad2-IRES-Cre mice were subsequently bred together for many generations (and the FLPe transgene was removed).
In 2010, Gad2-IRES-Cre knock-in mice on a mixed genetic background (mostly C57BL/6;129S4 ; with black coat color) were sent to The Jackson Laboratory Repository. Upon arrival, sperm was cryopreserved. An aliquot of that frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664) and rederive our live colony, which was thereafter maintained by breeding mutant mice together.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	When Gad2-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Gad2-expressing cells (GAD2 positive neurons) of the offspring.

Control Suggestions

Approximate Controls

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Selected References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

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Genetics

Gad2^tm2(cre)Zjh

Allele Symbol: Gad2^tm2(cre)Zjh

Allele Name	targeted mutation 2, Z Josh Huang
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Gad2^tm2(cre)Zjh; targeted mutation 2, Z Josh Huang
Gene Symbol and Name	Gad2, glutamic acid decarboxylase 2
Gene Synonym(s)	Gad-2; GAD(65); GAD65; 6330404F12Rik; 6330404F12Rik; RIKEN cDNA 6330404F12 gene; Gad-2; gad65
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	When Gad2-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Gad2-expressing cells (GAD2 positive neurons) of the offspring.
Strain of Origin	(C57BL/6 x 129S4/SvJae)F1
Chromosome	2
Molecular Note	A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the Gad2 (glutamic acid decarboxylase 2) gene. This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the selection cassette. The FLPe transgene was subsequently bred out of the background.
Mutations Made By	Z. Josh Huang, Cold Spring Harbor Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Genetics Research
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers: Cre-lox System
- Neurobiology Research
  - cell marker
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gad2^tm2(cre)Zjh/Gad2^tm2(cre)Zjh
involves: 129S4/SvJae * C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected
- Normal - mice are viable, fertile, normal in size and display no gross physical or behavioral abnormalities

References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

Additional References

Garcia-Junco-Clemente P; Ikrar T; Tring E; Xu X; Ringach DL; Trachtenberg JT. 2017. An inhibitory pull-push circuit in frontal cortex. Nat Neurosci 20(3):389-392PubMed: 28114295 MGI: J:271925

Additional - Gad2^tm2(cre)Zjh related

Technical Support

Contact Technical Support

Genotyping Protocols
- MELT: Gad2^tm2(cre)Zjh Alternate1
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

Homozygous mice are viable and fertile. When maintaining a live colony at The Jackson Laboratory Repository, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the Gad2-IRES-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #010802 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available Now

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Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Gad2<tm2(cre)Zjh>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Also Known As: Gad2-IRES-Cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Gad2tm2(cre)Zjh

Allele Symbol: Gad2tm2(cre)Zjh

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gad2tm2(cre)Zjh/Gad2tm2(cre)Zjhinvolves: 129S4/SvJae * C57BL/6 * SJL

normal phenotype

References

Additional References

Additional - Gad2tm2(cre)Zjh related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Gad2^tm2(cre)Zjh

Allele Symbol: Gad2^tm2(cre)Zjh

Genotype: Gad2^tm2(cre)Zjh/Gad2^tm2(cre)Zjh
involves: 129S4/SvJae * C57BL/6 * SJL

Additional - Gad2^tm2(cre)Zjh related