This targeted mutation of the (Trim21) gene displays enhanced proinflammatory cytokine expression patterns.
Herbert C. Morse III, Laboratory of Immunopathology, NIAID, NI
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Trim21 | tripartite motif-containing 21 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
EGFP expression is observed at high levels in lymph node, spleen, and thymus and at lower levels in kidney, heart and liver. Very low EGFP expression occurs in brain. Stimulated (LPS or polyI:C) embryonic fibroblasts derived from these mice exhibit enhanced expression of proinflammatory cytokines (TNFA, IL6 and CXCL10). A similar response is not observed in bone marrow dendritic cells or macrophages. Dendritic cells (CD11c+) generated from homozygous bone marrow are reduced by 25% as compared to controls, although, numbers of bone marrow macrophages are not altered. This mutant mouse strain may be useful in study of NFKB regulation and inflammatory responses.
A targeting vector was designed to insert the coding sequence of Enhanced Green Fluorescent Protein (EGFP) into exon 3 and a polyA signal and loxP-flanked PGK-neomycin resistance cassette downstream of exon 3. The construct was electroporated into C57BL/6 derived Bruce 4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were interbred and crossed to a C57BL/6J OzCre deleter mouse produced by Ozgene to excise the neo cassette leaving a single loxP site downstream of the EGFP in exon 3. Selected breeding eliminated Cre recombinase. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP expression is observed in lymph node, spleen, and thymus and at lower levels in kidney, heart, liver, and brain. |
Allele Name | targeted mutation 1, Herbert Morse III |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Trim21, tripartite motif-containing 21 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP expression is observed in lymph node, spleen, and thymus and at lower levels in kidney, heart, liver, and brain. |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 7 |
Molecular Note | Exons 3 through 5 were replaced with EGFP and a floxed neo cassette. Cre mediated recombination removed the neo cassette. |
Mutations Made By | Herbert Morse III, Laboratory of Immunopathology, NIAID, NI |
While maintaining a live colony, these mice are bred as homozygotes.
When using the C57BL/6-Trim21tm1Hm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010724 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Trim21<tm1Hm> |
Frozen Mouse Embryo | C57BL/6-Trim21<tm1Hm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Trim21<tm1Hm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Trim21<tm1Hm>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Trim21<tm1Hm>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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