This Snai2del1 mutant mouse strain may be useful in studies of Waardenburg Syndrome, Type IID, pigmentation, gonadal development and hematopoiesis.
Thomas Gridley, Maine Medical Center Research Institute
Mice that are homozygous for the targeted mutation are viable, subfertile, and are smaller in size than wildtype controls. Homozygotes have diluted coat color and areas of depigmentation, sometimes exhibiting white forehead blaze and spots on tails and feet. From birth to weaning age (approximately 3 weeks), homozygotes exhibit slowed growth rate and by 3 weeks of age weigh approximately 70% of wildtype control. After weaning, mutant growth rates are similar to wildtype, but mutants remain small in size. Homozygous adults develop eye infections (suppurative conjunctivitis and blepharitis). Homozygous males have reduced testes size due to reduced seminiferous tubules and are slightly subfertile, producing smaller litters sizes. Approximately 15% of homozygous males are infertile. Spermatogenesis is normal, however, in fertile homozygotes. Homozygotes exhibit macrocytic anemia, decreased hematocrit and leukocyte numbers. T cell differentiation is impaired and thymus size is diminished. Some mutant mice display hyperactivity and circling behavior.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the entire protein coding region of the gene. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. The mice were backcrossed to C57BL/6J once to establish the colony.
|Allele Name||targeted mutation 2, Tom Gridley|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Slug-; Slughdelta1; Snai2del1|
|Gene Symbol and Name||Snai2, snail family zinc finger 2|
|Gene Synonym(s)||SLUG; SLUGH; SLUGH1; SNAIL2; Slug; Slugh; Slugh; Snail2; WS2D; slug, chicken homolog|
|Strain of Origin||129S1/Sv-Oca2<+> Tyr<+> Kitl<+>|
|Molecular Note||The gene was inactivated by replacement of the entire protein coding region with a PGK-neo cassette via homologous recombination.|
|Mutations Made By|| |
Thomas Gridley, Maine Medical Center Research Institute
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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