C57BL/6-Ptrh2^tm1Eruo/J

Stock number: 010711
Research areas: Apoptosis Research, Research Tools

Description

This conditional mutation of the peptidyl-tRNA hydrolase 2 (Ptrh2) gene may be useful in generating conditional mutants to study of anoikis / apoptosis.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study anoikis /apoptosis.

When crossed to B6.129S4-Meox2^tm1(cre)Sor/J (see Stock No. 003755) Cre recombinase expression in early embryos results in postnatal lethality by P8-10.

Development

A targeting vector was designed to place a loxP site upstream of the targeted gene and a FRT-flanked neomycin resistance cassette followed by a loxP site downstream of the coding region. The construct was electroporated into C57BL/6 derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were intercrossed to produce homozygous mice. Progeny from this mating were crossed to C57BL/6 mice several times prior to arrival at The Jackson Laboratory.

Allele

Symbol: Ptrh2^tm1Eruo
Name: targeted mutation 1, Erkki Ruoslahti
MGI accession ID: MGI:3775073
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ptrh2
Marker name: peptidyl-tRNA hydrolase 2
Chromosome: 11
Strain of origin: B6.Cg-Thy1^a
Expressed genes: neo,neomycin resistance,bacterial
Molecular note: An frt-flanked neo cassette with a 3' loxP site was inserted downstream of the open reading frame and an additional loxP site was inserted upstream of the open reading frame. Mice were commercially prepared by Ozgene.