This conditional mutation of the peptidyl-tRNA hydrolase 2 (Ptrh2) gene may be useful in generating conditional mutants to study of anoikis / apoptosis.
Erkki Ruoslahti, Burnham Institute for Medical Research
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Ptrh2 | peptidyl-tRNA hydrolase 2 |
Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study anoikis /apoptosis.
When crossed to B6.129S4-Meox2tm1(cre)Sor/J (see Stock No. 003755) Cre recombinase expression in early embryos results in postnatal lethality by P8-10.
A targeting vector was designed to place a loxP site upstream of the targeted gene and a FRT-flanked neomycin resistance cassette followed by a loxP site downstream of the coding region. The construct was electroporated into C57BL/6 derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were intercrossed to produce homozygous mice. Progeny from this mating were crossed to C57BL/6 mice several times prior to arrival at The Jackson Laboratory.
Expressed Gene | neo, neomycin resistance, bacterial |
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Site of Expression |
Allele Name | targeted mutation 1, Erkki Ruoslahti |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Bit1flxNeo |
Gene Symbol and Name | Ptrh2, peptidyl-tRNA hydrolase 2 |
Gene Synonym(s) | |
Expressed Gene | neo, neomycin resistance, bacterial |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 11 |
Molecular Note | An frt-flanked neo cassette with a 3' loxP site was inserted downstream of the open reading frame and an additional loxP site was inserted upstream of the open reading frame. Mice were commercially prepared by Ozgene. |
Mutations Made By | Barry Rowan, Burnham Institute for Medical Research |
While maintaining a live colony, these mice are bred as homozygotes.
When using the C57BL/6-Ptrh2tm1Eruo/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010711 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ptrh2<tm1Eruo> |
Frozen Mouse Embryo | C57BL/6-Ptrh2<tm1Eruo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Ptrh2<tm1Eruo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Ptrh2<tm1Eruo>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Ptrh2<tm1Eruo>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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