These mice possess loxP sites on either side of exon 2 of the targeted Adora2a gene and may be useful in generating conditional mutations for studying locomoter activity response to addictive substances.
Joel Linden, University of Virginia
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the critical exon deleted in the cre-expressing tissues.
When bred to a strain expressing Cre recombinase in the forebrain (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of locomoter activity response to addictive substances.
A loxP site-flanked targeting vector was used to insert loxP sites flanking exon 2. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Resulting chimeric male animals were backcrossed to wildtype C57BL/6J mice for 2 generations using a marker assisted protocol. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J once to establish the colony.
|Allele Name||targeted mutation 1, Yuan-Ji Day|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Allele Synonym(s)||A2Aflox; A2ARflox; Adora2atm1Masc; Adora2atmJyd|
|Gene Symbol and Name||Adora2a, adenosine A2a receptor|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Exon 2, a critical exon, was flanked by loxP sites.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129-Adora2atm1Dyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010687 in your Materials and Methods section.