These Runx1f/f (Runx1, runt related transcription factor 1) mutant mice may be useful in generating conditional mutations for studying progenitor and stem cells, hematopoiesis and development, nociception and immunology.
Nancy Speck, University of Pennsylvania
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissues.
When bred to a strain with inducible Cre recombinase during development (see Stock No. 003556 for example), this mutant mouse strain may be useful in studies of hematopoiesis and development.
A loxP site flanked targeting vector containing a PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, then the resulting F1 heterozygotes intercrossed to generate homozygotes carrying the floxed allele. These mice were then crossed to transgenic mice expressing Cre recombinase (carrying the Tg(Cdh5-cre)1Spe, VEC-cre, transgene on a mixed B6;129 background). A male mouse that retained the loxP site flanked exon 4 and no longer contained the neo selection cassette was then bred to a C57BL/6J female. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J once to establish the colony.
|Allele Name||targeted mutation 3.1, Nancy A Speck|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||targeted mutation 3.1, Nancy A Speck; Runx1tm3.1Spe|
|Gene Symbol and Name||Runx1, runt related transcription factor 1|
|Gene Synonym(s)||core binding factor alpha 2; Cbfa2; AML1; AMLCR1; runt domain, alpha subunit 2; CBFA2; EVI-1; AML1-EVI-1; AI462102; Pebp2a2; PEBP2aB; expressed sequence AI462102; PEBP2-alpha; CBF-alpha-2; B; PEA2-alpha; Cbfa2; Aml1; PEBP2alpha; CBF2alpha|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A loxP site flanked targeting vector containing PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Chimeras were crossed to C57BL/6NTac mice for 3 generations then made homozygous. The mice were then crossed to (B6129S)F1/J with the resulting F2 intercrossed to generate homozygotes carrying the floxed allele. These mice were then crossed to transgenic mice expressing Cre recombinase (carrying the Tg(Cdh5-cre)1Spe, (VEC-cre) transgene on a mixed B6;129 background.|
|Mutations Made By|| |
Nancy Speck, University of Pennsylvania
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129-Runx1tm3.1Spe/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010673 in your Materials and Methods section.
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