This transgenic line expresses the human RAC1 gene carrying the constitutively active G12V mutation and displays hypertension, accelerated wound healing and Kaposi's sarcoma-like tumors.
Pascal J. Goldschmidt-Clermont, University of Miami Miller School of Med
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice that are homozygous for the transgene are viable, fertile and normal in size. Males develop reddish, discolored tail lesions beginning at 8 months of age. Females develop tumors at approximately 18 months. Tumors exhibit many characteristics of Kaposi's sarcoma including an increase in spindle cells without identifiable vascular structures, slits containing red blood cells and hemosiderin between cells, expression of phenotypic markers (such as CD31, CD34, and von Willebrand factor), and an association with male gender. Transgenic mice exhibit moderate hypertension, left ventricular hypertrophy, accelerated wound healing and increased vascular formation in response to wound grafting. This mutant mouse strain may be useful in studies of blood pressure, angiogenesis, wound healing, oxidative stress and Kaposi's sarcoma.
A transgenic construct containing the constitutively active glycine to valine mutation at amino acid 12 (G12V) of the human RAC1 gene under the control of mouse smooth muscle alpha actin (Acta2) promoter. This transgene was microinjected into single-cell stage FVB/N pronuclei. Founder line 33 was subsequently established. The mice were then crossed to FVB/N by the donating laboratory.
Expressed Gene | RAC1, Rac family small GTPase 1, human |
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Site of Expression |
Allele Name | transgene insertion 33, Pascal J Goldschmidt-Clermont |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | RacCA |
Gene Symbol and Name | Tg(Acta2-RAC1*G12V)33Pjgc, transgene insertion 33, Pascal J Goldschmidt-Clermont |
Gene Synonym(s) | |
Promoter | Acta2, actin, alpha 2, smooth muscle, aorta, mouse, laboratory |
Expressed Gene | RAC1, Rac family small GTPase 1, human |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The cDNA coding for human RAC1 was mutated with a glycine 12 to valine substitution. The cDNA of RacCA, including its polyadenylation tail, was cloned within the Smp-8 plasmid that contained a 3.6 kb segment of the 5 kb-region of SMA promoter. The SMA promoter was used to induce selective overexpression of RacCA in SMCs. The purified fragment was microinjectedinto fertilized mouse eggs from FVB/N females, |
While maintaining a live colony, these mice are bred as homozygotes.
When using the FVB/N-Tg(Acta2-RAC1*G12V)33Pjgc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010634 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Acta2-RAC1*G12V)33Pjgc |
Frozen Mouse Embryo | FVB/N-Tg(Acta2-RAC1*G12V)33Pjgc/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Acta2-RAC1*G12V)33Pjgc/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Acta2-RAC1*G12V)33Pjgc/J | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(Acta2-RAC1*G12V)33Pjgc/J | $3373.50 |
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