B6;SJL-Tg(tetO-Erbb2*)8-4Jek/J

Stock number: 010577
Product name: TetRE-ErbB2 , TRE-ErbB2 , TRE-Neu , TRE-Neu-SV40
Strain synonyms: TetRE-ErbB2*V664E , TRE-ErbB2*V664E , TRE-Neu*V664E , TRE-Neu*V664E-SV40 , Tet-ErbB2*V664E , TetRE-ErbB2^V664E , TRE-ErbB2^V664E , TRE-Neu^V664E , TRE-Neu^V664E-SV40 , Tet-ErbB2^V664E
Research areas: Cancer Research, Cell Biology Research, Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Neurobiology Research, Research Tools

Description

These TetRE-ErbB2 (TRE-ErbB2 or TRE-Neu) transgenic mice express an activated form of the rat ErbB2 regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. The amino acid mutation within the transmembrane domain of the ErbB2 receptor protein kinase facilitates its oligomerization and activation independent of a ligand; resulting in hyperplasia in tissues where it is expressed. These TetRE-ErbB2 mice may be useful in generating bi-transgenic mutant mice for the conditional (inducible/reversible) expression of a constitutively active ErbB2 isoform, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins).

Detailed description

The donating investigator reports that homozygous mice are viable and fertile. These TetRE-ErbB2 (TRE-ErbB2 or TRE-Neu) transgenic mice have expression of an activated form of the rat ErbB2 regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of the constitutively active ErbB2 protein may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring.

The amino acid mutation within the transmembrane domain of the ErbB2 receptor protein kinase facilitates its oligomerization and activation independent of a ligand; resulting in hyperplasia in tissues where it is expressed.

These TetRE-ErbB2 mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of an activated ErbB2 isoform, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins).

Development

The TetRE-ErbB2 (pTRE-Neu-SV40) transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 4.8 kb cDNA sequence from the activated form of rat c-neu/erbB2 (ErbB2; with Val to Glu mutation within the transmembrane domain at amino acid 664), and an SV40 intron/polyA sequence. This 6.8 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 8-4 were bred with wildtype sibling mice to establish the colony. The donating investigator reports that TetRE-ErbB2 transgenic mice were bred together, and/or to tTA-transgenic mice (on the same mixed C57BL/6;SJL genetic background) for many generations. Mice harboring only the TetRE-ErbB2 transgene were then sent to The Jackson Laboratory. Upon arrival, transgenic mice were bred with B6SJLF1/J hybrid mice (Stock No. 100012) for at least one generation to establish this colony.

Allele

Symbol: Tg(tetO-Erbb2*)8-4Jek
Name: transgene insertion 8-4, Jeffrey E Kudlow
MGI accession ID: MGI:3850387
Generation method: Transgenic
Attributes: Inducible; Constitutively active; Inserted expressed sequence
Synonyms: TetRE-ErbB2; TRE-ErbB2; TRE-Neu
Marker symbol: Tg(tetO-Erbb2*)8-4Jek
Marker name: transgene insertion 8-4, Jeffrey E Kudlow
Marker synonyms: TetRE-ErbB2; TRE-ErbB2; TRE-Neu
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)
Expressed genes: Erbb2,erb-b2 receptor tyrosine kinase 2,rat
Molecular note: The TetRE-ErbB2 (pTRE-Neu-SV40) transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 4.8 kb cDNA sequence from the activated form of rat c-neu/erbB2 (ErbB2; with Val to Glu mutation within the transmembrane domain at amino acid 664), and an SV40 intron/polyA sequence. This 6.8 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 8-4 were bred with wildtype sibling mice to establish the colony.