The mouse Penk (preproenkephalin) promoter drives expression of the C. elegans glutamate-gated chloride channel beta subunit and cre/ESR1. When crossed with a strain containing a loxP site-flanked sequence of interest, the flanked sequences are deleted in the offspring upon administration of tamoxifen.
David J Anderson, California Institute of Technology
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
In this transgenic line, a mouse Penk (preproenkephalin) promoter drives expression of the C. elegans glutamate-gated chloride channel beta subunit for recombinatorial, inducible, reversible neuronal silencing (Lerchner, et al. Neuron 54(1):35-49. 2007) and cre/ESR1. Cre recombinase is fused to a mutant form of the mouse estrogen receptor ligand binding domain. Restricted to the cytoplasm, the fusion protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the flanked sequences are deleted in the offspring upon administration of tamoxifen.
The beta subunit of ivermectin-sensitive C. elegans glutamate-gated choride channels (glc-2), followed by IRES and cre/ESR1 sequences was inserted at the ATG start codon of the mouse preproenkephalin (Penk) BAC RP23-438D19. The transgenic vector was introduced to FVB embryos and the resultant mice were backcrossed five times to C57BL/6N by the donating laboratory.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | neurons |
Allele Name | transgene insertion 2, David J Anderson |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Enk:GluCl-beta-iCreER |
Gene Symbol and Name | Tg(Penk-glc-2-cre/ERT2)2And, transgene insertion 2, David J Anderson |
Gene Synonym(s) | |
Promoter | Penk, preproenkephalin, mouse, laboratory |
Promoter | glc-2, glutamate-gated chlorided channel, betaa subunit, C. elegans |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | neurons |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | The beta subunit of ivermectin-sensitive C. elegans glutamate-gated choride channels (glc-2), followed by IRES and cre/ERT2 sequences was inserted at the ATG start codon of the mouse preproenkephalin (Penk) BAC RP23-438D19. The transgenic vector was introduced to FVB embryos. |
Mutations Made By | David Anderson, California Institute of Technology |
When maintained as a live colony, hemizygotes may be bred.
When using the B6N.FVB-Tg(Penk-glc-2-cre/ERT2)2And/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010550 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Penk-glc-2-cre/ERT2)2And |
Frozen Mouse Embryo | B6N.FVB-Tg(Penk-glc-2-cre/ERT2)2And/J | $2595.00 |
Frozen Mouse Embryo | B6N.FVB-Tg(Penk-glc-2-cre/ERT2)2And/J | $2595.00 |
Frozen Mouse Embryo | B6N.FVB-Tg(Penk-glc-2-cre/ERT2)2And/J | $3373.50 |
Frozen Mouse Embryo | B6N.FVB-Tg(Penk-glc-2-cre/ERT2)2And/J | $3373.50 |
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