B6N.FVB-Tg(Penk-glc-2-cre/ERT2)2And/J

Stock number: 010550
Research areas: Research Tools

Description

The mouse Penk (preproenkephalin) promoter drives expression of the C. elegans glutamate-gated chloride channel beta subunit and cre/ESR1. When crossed with a strain containing a loxP site-flanked sequence of interest, the flanked sequences are deleted in the offspring upon administration of tamoxifen.

Detailed description

In this transgenic line, a mouse Penk (preproenkephalin) promoter drives expression of the C. elegans glutamate-gated chloride channel beta subunit for recombinatorial, inducible, reversible neuronal silencing (Lerchner, et al. Neuron 54(1):35-49. 2007) and cre/ESR1. Cre recombinase is fused to a mutant form of the mouse estrogen receptor ligand binding domain. Restricted to the cytoplasm, the fusion protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the flanked sequences are deleted in the offspring upon administration of tamoxifen.

Development

The beta subunit of ivermectin-sensitive C. elegans glutamate-gated choride channels (glc-2), followed by IRES and cre/ESR1 sequences was inserted at the ATG start codon of the mouse preproenkephalin (Penk) BAC RP23-438D19. The transgenic vector was introduced to FVB embryos and the resultant mice were backcrossed five times to C57BL/6N by the donating laboratory.

Allele

Symbol: Tg(Penk-glc-2-cre/ERT2)2And
Name: transgene insertion 2, David J Anderson
MGI accession ID: MGI:3849454
Generation method: Transgenic
Attributes: Recombinase-expressing; Inducible
Marker symbol: Tg(Penk-glc-2-cre/ERT2)2And
Marker name: transgene insertion 2, David J Anderson
Chromosome: UN
Strain of origin: FVB
Expressed genes: cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: neurons
Molecular note: The beta subunit of ivermectin-sensitive C. elegans glutamate-gated chloride channels (glc-2), followed by IRES and cre/ERT2 sequences was inserted at the ATG start codon of the mouse preproenkephalin (Penk) BAC RP23-438D19. The transgenic vector was introduced to FVB embryos.