These Notch3d1 mutant mice exhibit abnormal arterial wall morphology. This mutant mouse strain may be useful in studies of the Notch pathway during development, vascular development and angiogenesis.
Of note, the Notch3d1 allele is available with different amounts of C57BL/6J genome: Stock No. 010547 is three or more generations backcrossed onto C57BL/6J, whereas Stock No. 018910 is on a mixed C57BL/6J;129S1 genetic background.
Thomas Gridley, Maine Medical Center Research Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Notch3 | notch 3 |
Mice that are homozygous for the Notch3d1 targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern blot analysis of brain, lung and heart tissue or Western blot analysis of lung and brain tissue from homozygotes. Homozygotes exhibit disorganized artery wall morphology, and thin vascular smooth muscle cell coat and processes.
A targeting vector containing a PGK-neo cassette was used to delete 2.5kb of sequence encoding the EGF repeats 8 through 12. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric males were bred to C57BL/6J females for germline transmission. The Notch3d1 colony was then backcrossed to C57BL/6J inbred mice (Stock No. 000664) for three generations prior to sending to The Jackson Laboratory Repository in 2010 as Stock No. 010547. To establish our live colony, mice were bred to C57BL/6J inbred mice. In 2010, sperm was frozen at generation N3. As of 2016, Stock No. 010547 is available from the sperm frozen in 2010.
In January 2014, the live colony was bred once to B6129SF1/J mice (Stock No. 101043). However, this does not affect the genetic background of the Stock No. 010547 available at this time (2016).
Allele Name | targeted mutation 1, Tom Gridley |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Notch3d1 |
Gene Symbol and Name | Notch3, notch 3 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 17 |
Molecular Note | A 2.5 kb genomic fragment containing sequence encoding EGF repeats 8 through 12 was replaced with a PGK-neo cassette inserted by homologous recombination. The absence of transcript and protein was indicated by Northern and Western blot analysis of homozygous mutant mice. |
Mutations Made By | Thomas Gridley, Maine Medical Center Research Institute |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Notch3d1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #010547 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Notch3<tm1Grid> |
Frozen Mouse Embryo | B6;129S1-Notch3<tm1Grid>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S1-Notch3<tm1Grid>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S1-Notch3<tm1Grid>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S1-Notch3<tm1Grid>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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