This targeted mutation of cAMP responsive element binding protein 3-like 3 (Creb3l3) gene exhibits a reduction of acute phase response proteins following ER stress and may be useful in studies of the systemic inflammatory response to ER stress.
Stephen Duncan, Medical College of Wisconsin
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Creb3l3 | cAMP responsive element binding protein 3-like 3 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In response to endoplasmic reticulum (ER) stress induced by tunicamycin, homozygous mice exhibit a reduced expression of the acute phase response proteins C-reactive protein, serum amyloid P-component and serum amyloid A3. This mutant mouse strain may be useful in studies of the systemic inflammatory response to ER stress.
A targeting vector was designed to place a loxP site between exons 11 and 12 and FRT-flanked neomycin resistance cassette with a loxP site between exons 3 and 4. The construct was electroporated into 129S1/SvImJ and 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to CD1 mice. To remove the floxed neo cassette and exons 4 through 11, mice were crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (Stock #003724). Mice retain a single loxP site 3 prime to exon 3. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Allele Name | targeted mutation 1.1, Stephen A Duncan |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CrebH- |
Gene Symbol and Name | Creb3l3, cAMP responsive element binding protein 3-like 3 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv or 129X1/SvJ |
Chromosome | 10 |
General Note | ES cell line = Jxl1. |
Molecular Note | A loxP site and a frt-flanked neomycin resistance cassette were inserted between exons 3 and 4, and a second loxP site was inserted between exons 11 and 12. The floxed region included the neo cassette. Mice were crossed with mice carrying Tg(Ella-cre)C5379Lmgd to remove exons 4 through 11 and parts of introns 3 and 11. One loxP site remains downstream of exon 3. Southern blot and PCR analyses on genomic DNA confirmed that the correct targeting and recombination events occurred. |
Mutations Made By | Stephen Duncan, Medical College of Wisconsin |
While maintaining a live colony, these mice are bred as homozygotes.
When using the STOCK Creb3l3tm1.1Sad/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010538 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Creb3l3<tm1.1Sad> |
Frozen Mouse Embryo | STOCK Creb3l3<tm1.1Sad>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Creb3l3<tm1.1Sad>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Creb3l3<tm1.1Sad>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Creb3l3<tm1.1Sad>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.