STOCK Creb3l3^tm1.1Sad/J

Stock number: 010538
Research areas: Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

This targeted mutation of cAMP responsive element binding protein 3-like 3 (Creb3l3) gene exhibits a reduction of acute phase response proteins following ER stress and may be useful in studies of the systemic inflammatory response to ER stress.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In response to endoplasmic reticulum (ER) stress induced by tunicamycin, homozygous mice exhibit a reduced expression of the acute phase response proteins C-reactive protein, serum amyloid P-component and serum amyloid A3. This mutant mouse strain may be useful in studies of the systemic inflammatory response to ER stress.

Development

A targeting vector was designed to place a loxP site between exons 11 and 12 and FRT-flanked neomycin resistance cassette with a loxP site between exons 3 and 4. The construct was electroporated into 129S1/SvImJ and 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to CD1 mice. To remove the floxed neo cassette and exons 4 through 11, mice were crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (Stock #003724). Mice retain a single loxP site 3 prime to exon 3. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Allele

Symbol: Creb3l3^tm1.1Sad
Name: targeted mutation 1.1, Stephen A Duncan
MGI accession ID: MGI:3811836
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Creb3l3
Marker name: cAMP responsive element binding protein 3-like 3
Chromosome: 10
Strain of origin: 129S1/Sv or 129X1/SvJ
Molecular note: A loxP site and a frt-flanked neomycin resistance cassette were inserted between exons 3 and 4, and a second loxP site was inserted between exons 11 and 12. The floxed region included the neo cassette. Mice were crossed with mice carrying Tg(Ella-cre)C5379Lmgd to remove exons 4 through 11 and parts of introns 3 and 11. One loxP site remains downstream of exon 3. Southern blot and PCR analyses on genomic DNA confirmed that the correct targeting and recombination events occurred.
General note: ES cell line = Jxl1.