These targeted mutant mice carry tamoxifen-inducible Cre under the transcriptional control of the mouse Bmi1 (Bmi1 polycomb ring finger oncogene) promoter. When crossed with a strain containing a loxP-flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions, and when crossed with a floxed reporter strain, lineage tracing of Bmi1-expressing cells is possible.
Dr. Mario R Capecchi, University of Utah
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing, Inducible) | Bmi1 | Bmi1 polycomb ring finger oncogene |
These targeted mutant mice carry tamoxifen-inducible Cre under the transcriptional control of the mouse Bmi1 (Bmi1 polycomb ring finger oncogene) promoter specifically expressed in discrete cells located near the bottom of crypts in the small intestine (predominantly four cells above the base of the crypt). When crossed with a strain containing a loxP-flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. When crossed with a floxed reporter strain, lineage tracing of Bmi1-expressing cells is possible.
An IRES (internal ribosome entry site)-Cre-ER (estrogen receptor binding domain) fusion and FRT-flanked neomycin resistance cassette was inserted between the stop codon and the first putative polyadenylation site of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Resultant heterozygotes were crossed with a Flpe deleter mouse to remove the FRT-flanked neomycin cassette. This strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
---|---|
Site of Expression | Bmi1 is expressed in discrete cells located near the bottom of crypts in the small intestine, predominantly four cells above the base of the crypt (+4 position). |
Allele Name | targeted mutation 1, Mario R Capecchi |
---|---|
Allele Type | Targeted (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Bmi1CreER; Bmi1tm1(cre/ESR1)Mrc |
Gene Symbol and Name | Bmi1, Bmi1 polycomb ring finger oncogene |
Gene Synonym(s) | |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | Bmi1 is expressed in discrete cells located near the bottom of crypts in the small intestine, predominantly four cells above the base of the crypt (+4 position). |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 2 |
Molecular Note | An IRES followed by a cre/ERT fusion and an frt-flanked neo cassette was inserted downstream of the translation stop codon. Germ line, flp-mediated recombination was used to remove the neo cassette. |
Mutations Made By | Dr. Mario Capecchi, University of Utah |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the Bmi1Cre-ER mouse strain in a publication, please cite the originating article(s) and include JAX stock #010531 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Bmi1<tm1(cre/ESR1)Mrc> |
Frozen Mouse Embryo | B6;129-Bmi1<tm1(cre/ERT)Mrc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Bmi1<tm1(cre/ERT)Mrc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Bmi1<tm1(cre/ERT)Mrc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Bmi1<tm1(cre/ERT)Mrc>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.