B6;129-Gt(ROSA)26Sor^tm1(DTA)Mrc/J

Stock number: 010527
Product name: Rosa26-DTA176
Research areas: Research Tools

Description

These R26R^DTA (or ROSA26-DTA176) mice carry a loxP-flanked stop cassette associated with an attentuated diptheria toxin. When crossed with a Cre recombinase-expressing strain, tissue/cell-specific ablation of cells can be achieved.

Detailed description

These R26R^DTA (or ROSA26-DTA176) mice carry a loxP-flanked stop cassette associated with an attentuated diptheria toxin. When crossed with a Cre recombinase-expressing strain, tissue/cell-specific ablation of cells can be achieved. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

Development

A loxP-flanked stop cassette followed by the coding sequence for an attenuated diphtheria toxin A chain (tox 176) were introduced to the Gt(ROSA)26Sor gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1-45 embryonic stem (ES) cells. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.

Allele

Symbol: Gt(ROSA)26Sor^tm1(DTA)Mrc
Name: targeted mutation 1, Mario R Capecchi
MGI accession ID: MGI:3618991
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Inserted expressed sequence; No functional change
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: Dta,Diphtheria toxin A chain,
Site of expression: Attentuated diptheria toxin (DTA176) is expressed in cre-expressing tissues after breeding with a strain carrying cre recombinase.
Molecular note: The DTA176 coding sequence was targeted into the Rosa26 locus but remains unexpressed until a loxP flanked stop sequence is removed via cre-mediated recombination.