These R26RDTA (or ROSA26-DTA176) mice carry a loxP-flanked stop cassette associated with an attentuated diptheria toxin. When crossed with a Cre recombinase-expressing strain, tissue/cell-specific ablation of cells can be achieved.
Dr. Mario R Capecchi, University of Utah
Genetic Background | Generation |
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F?+N1F23
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, No functional change) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These R26RDTA (or ROSA26-DTA176) mice carry a loxP-flanked stop cassette associated with an attentuated diptheria toxin. When crossed with a Cre recombinase-expressing strain, tissue/cell-specific ablation of cells can be achieved. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
A loxP-flanked stop cassette followed by the coding sequence for an attenuated diphtheria toxin A chain (tox 176) were introduced to the Gt(ROSA)26Sor gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1-45 embryonic stem (ES) cells. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
Expressed Gene | Dta, Diphtheria toxin A chain, |
---|---|
Site of Expression | Attentuated diptheria toxin (DTA176) is expressed in cre-expressing tissues after breeding with a strain carrying cre recombinase. |
Allele Name | targeted mutation 1, Mario R Capecchi |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, No functional change) |
Allele Synonym(s) | R26RDTA; ROSADTA; ROSA26-DTA176 |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | Dta, Diphtheria toxin A chain, |
Site of Expression | Attentuated diptheria toxin (DTA176) is expressed in cre-expressing tissues after breeding with a strain carrying cre recombinase. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 6 |
Molecular Note | The DTA176 coding sequence was targeted into the Rosa26 locus but remains unexpressed until a loxP flanked stop sequence is removed via cre-mediated recombination. |
Mutations Made By | Dr. Mario Capecchi, University of Utah |
When maintained as a live colony, homozygotes may be bred.
When using the Rosa26-DTA176 mouse strain in a publication, please cite the originating article(s) and include JAX stock #010527 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1(DTA)Mrc> |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(DTA)Mrc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(DTA)Mrc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(DTA)Mrc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(DTA)Mrc>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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