These Pygo2 -floxed mice may be useful in generating conditional mutations to study lens development.
Richard A. Lang, Cincinnati Children's Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Pygo2 | pygopus 2 |
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in eye tissues results in microophthalmia and abnormal lens induction. This mutant mouse strain is useful in studies of lens development, but may also be useful in studies of other organs as well.
For example, when crossed to a strain with widespread Cre recombinase expression (see Stock No. 003465 and 006054), this mutant mouse strain may be useful in lens development.
When crossed to a strain expressing Cre recombinase in midbrain and spinal cord (see Stock No. 007807), this mutant mouse strain may be useful in studies of lens development.
A targeting vector was designed to place a loxP site upstream of exon 3, and a frt-flanked Pgk-Neo cassette and loxP site donstream of exon 3 of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were used to generate chimeric males. Chimeric males may or may not have been bred with Swiss Black mice. At some point, the mice were bred to a FLP recombinase-expressing strain to remove the frt-flanked Pgk-Neo cassette. These mice were maintained by intercrossing prior to sending to The Jackson Laboratory. Agouti mice were received. Upon arrival, the mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, S Steven Potter |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Pygo2flox; Pygo2tm1Lan |
Gene Symbol and Name | Pygo2, pygopus 2 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 3 |
Molecular Note | LoxP sites were inserted to flank exon 3. |
Mutations Made By | Richard Lang, Cincinnati Children's Hospital |
When maintaining a live colony these mice are bred as homozygotes.
When using the STOCK Pygo2tm1.1Ssp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010499 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Pygo2<tm1Lan> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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