Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in eye tissues results in microophthalmia and abnormal lens induction. This mutant mouse strain is useful in studies of lens development, but may also be useful in studies of other organs as well.
When crossed to a strain expressing Cre recombinase in midbrain and spinal cord (see Stock No. 007807), this mutant mouse strain may be useful in studies of lens development.
A targeting vector was designed to place a loxP site upstream of exon 3, and a frt-flanked Pgk-Neo cassette and loxP site donstream of exon 3 of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were used to generate chimeric males. Chimeric males may or may not have been bred with Swiss Black mice. At some point, the mice were bred to a FLP recombinase-expressing strain to remove the frt-flanked Pgk-Neo cassette. These mice were maintained by intercrossing prior to sending to The Jackson Laboratory. Agouti mice were received. Upon arrival, the mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, S Steven Potter|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Pygo2flox; Pygo2tm1Lan|
|Gene Symbol and Name||Pygo2, pygopus 2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||LoxP sites were inserted to flank exon 3.|
|Mutations Made By|| |
Richard Lang, Cincinnati Children's Hospital
When maintaining a live colony these mice are bred as homozygotes.
When using the STOCK Pygo2tm1.1Ssp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010499 in your Materials and Methods section.