These Clock (circadian locomoter output cycles kaput), Clockflox, mutant mice may be useful in generating conditional mutations for studying circadian rhythm and behavior.
David R. Weaver, Univ of Massachusetts Medical School
These mice possess loxP sites on either side of exons 5 and 6 of the targeted gene. Mice that are homozygous for this conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5 and 6 deleted in the cre-expressing tissue(s).
When bred to a strain expressing Cre recombinase in the male germ line (see Stock No. 003328 for example), this mutant mouse strain may be useful in studies of circadian rhythm and behavior.
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 5 of the targeted gene, and another loxP site was inserted downstream of exon 6. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 6 generations. Upon arrival at The Jackson Laboratory, the mice were crossed with C57BL/6J once to establish the colony.
|Allele Name||targeted mutation 1, Steven M Reppert|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Clockflox; Clocktm1Drw|
|Gene Symbol and Name||Clock, circadian locomotor output cycles kaput|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A floxed neo cassette containing neomycin resistance and thymidine kinase genes was inserted upstream of exon 5 and a loxp site was inserted downstream of exon 6. Transient cre expression in ES cells removed the neo cassette leaving exons 5 and 6 floxed|
|Mutations Made By|| |
Steven Reppert, University of Massachusetts Medical Scho
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S4-Clocktm1Rep/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010490 in your Materials and Methods section.