These Csnk1d (casein kinase 1, delta), CK1deltaflox, mutant mice may be useful in generating conditional mutations for studying circadian rhythm and behavior.
David R. Weaver, Univ of Massachusetts Medical School
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. After Cre-mediated excision of exon 2, a mutant gene product (protein) is detected by Western blot analysis of liver tissue. This mutant protein is relatively unstable, does not interact with PER (Period) proteins, and does not have biological activity with respect to PER protein phosphorylation. This mutant mouse strain may be useful in generating conditional mutations for studying circadian rhythm and behavior.
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6 for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed with C57BL/6J once to establish the colony.
|Allele Name||targeted mutation 1, David R Weaver|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Csnk1d, casein kinase 1, delta|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A loxP site was inserted upstream of exon 2 and a floxed neo cassette was inserted downstream of exon 2. Transient cre expression in ES cells removed the neo cassette leaving exon 2 floxed.|
|Mutations Made By|| |
David Weaver, Univ of Massachusetts Medical School
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S4-Csnk1dtm1Drw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010487 in your Materials and Methods section.