B6.129P2-Isg15^tm1Kpk/J

Stock number: 010486
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools, Virology Research

Description

This targeted mutation of the ISG15 ubitquitin-like modifier (Isg15) gene displays increased susceptibility to Influenza A/B and HSV-1, but LCMV or VSV and may be useful in studies of interferon-induced antiviral responses and differing susceptibilities to viral infection.

Detailed description

Mice homozygous for this allele are viable and fertile and possess a loxP site flanked neomycin resistance cassette disrupting exon 2 of the targeted gene. Composition of the major cell populations of the immune system in spleen, bone marrow, lymph nodes and thymus is not affected by the mutation. Homozygous mice have an increased susceptibility to influenza A/B, HSV-1 and Sinbis virus, however, response to VSV and LCMV is unaltered from control mice. These mice may be useful in studying interferon-induced antiviral responses and differing susceptibilities to viral infection.

Development

A targeting vector was used to insert a loxP-flanked neomycin cassette in exon 2, replacing the complete coding region. The construct was electroporated into 129P2/OlaHsd derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 for 10 generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Allele

Symbol: Isg15^tm1Kpk
Name: targeted mutation 1, Klaus-Peter Knobeloch
MGI accession ID: MGI:3586990
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Isg15
Marker name: ISG15 ubiquitin-like modifier
Chromosome: 4
Strain of origin: 129P2/OlaHsd
Molecular note: A floxed PGK-neo cassette replaced the complete coding region. Northern blot of mutant bone marrow failed to detect transcript. Western blot failed to detect protein in mutants.