This targeted mutation of the ISG15 ubitquitin-like modifier (Isg15) gene displays increased susceptibility to Influenza A/B and HSV-1, but LCMV or VSV and may be useful in studies of interferon-induced antiviral responses and differing susceptibilities to viral infection.
Klaus-Peter Knobeloch, University Freiburg
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Isg15 | ISG15 ubiquitin-like modifier |
Mice homozygous for this allele are viable and fertile and possess a loxP site flanked neomycin resistance cassette disrupting exon 2 of the targeted gene. Composition of the major cell populations of the immune system in spleen, bone marrow, lymph nodes and thymus is not affected by the mutation. Homozygous mice have an increased susceptibility to influenza A/B, HSV-1 and Sinbis virus, however, response to VSV and LCMV is unaltered from control mice. These mice may be useful in studying interferon-induced antiviral responses and differing susceptibilities to viral infection.
A targeting vector was used to insert a loxP-flanked neomycin cassette in exon 2, replacing the complete coding region. The construct was electroporated into 129P2/OlaHsd derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 for 10 generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Allele Name | targeted mutation 1, Klaus-Peter Knobeloch |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ISG15- |
Gene Symbol and Name | Isg15, ISG15 ubiquitin-like modifier |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 4 |
Molecular Note | A floxed PGK-neo cassette replaced the complete coding region. Northern blot of mutant bone marrow failed to detect transcript. Western blot failed to detect protein in mutants. |
Mutations Made By | Klaus-Peter Knobeloch, University Freiburg |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129P2-Isg15tm1Kpk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010486 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for sg15<tm1Kpk> |
Frozen Mouse Embryo | B6.129P2-Isg15<tm1Kpk>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Isg15<tm1Kpk>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Isg15<tm1Kpk>/J | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Isg15<tm1Kpk>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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