These LSL-HIF1dPA mice conditionally express a form of hemagglutinin (HA)-tagged human HIF1A (hypoxia inducible factor 1, alpha subunit; HIF1α HIF1dPA) cDNA that escapes recognition by the von Hippel-Lindau tumor suppressor protein by virtue of proline to alanine substitutions. When crossed with a tissue-specific cre strain, excision of a floxed stop cassette enables expression of the cDNA driven by the endogenous mouse Gt(ROSA)26Sor promoter. This strain may be useful in studies of von Hippel-Lindau disease mechanisms.
William G. Kaelin, Dana Farber Cancer Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These LSL-HIF1dPA mice conditionally express a form of hemagglutinin (HA)-tagged human HIF1A (HIF1&alpha, HIF1dPA) cDNA that escapes recognition by the von Hippel-Lindau tumor suppressor protein by virtue of proline to alanine substitutions. When crossed with a tissue-specific cre strain, excision of a floxed stop cassette enables expression of the cDNA driven by the endogenous mouse Gt(ROSA)26Sor promoter. There is no expression until the mice are exposed to Cre recombinase. This strain may be useful in studies of von Hippel-Lindau disease mechanisms.
For example, when crossed to a strain expressing Cre recombinase in liver (see Stock No. 003574), this mutant mouse strain may be useful in studies of VHL disease.
A loxP-flanked neomycin resistance cassette followed by hemagglutinin-tagged human HIF1A cDNA modified with two proline to alanine subsitutions (P402A, P564A) was introduced 3' of the mouse Gt(ROSA)26Sor promoter. The targeting vector was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. The line was backcrossed to BALB/c for three generations, then backcrossed four times to C57BL/6 by the donating laboratory.
Expressed Gene | HIF1A, hypoxia inducible factor 1 subunit alpha, human |
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Expressed Gene | HA, influenza hemagglutinin, |
Site of Expression |
Allele Name | targeted mutation 3, William G Kaelin |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | ROSA26-HA-HIF1dPA |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | HIF1A, hypoxia inducible factor 1 subunit alpha, human |
Expressed Gene | HA, influenza hemagglutinin, |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 6 |
Molecular Note | DNA encoding influenza virus hemagglutinin A- (HA-) tagged human hypoxia-inducible factor 1 alpha (HIF1alpha) with nucleotide substitutions leading to replacement of proline by alanine at amino acid positions 402 and 564 (P402A;P564A), preceded by a floxed neo-STOP cassette, was inserted into the targeted locus. The STOP sequence must be removed by Cre-mediated recombination to allow for expression of the mutated coding sequence. The amino acid substitutions prevent oxygen-dependent prolyl hydroxylation at a.a. position 402 or 564, which is required for binding by von Hippel-Lindau tumor suppressor (VHL). |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129S6(C)-Gt(ROSA)26Sortm3(HIF1A*)Kael/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009673 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gt(ROSA)26Sor<tm3(HIF1A*)Kael> |
Frozen Mouse Embryo | B6.129S6(C)-Gt(ROSA)26Sor<tm3(HIF1A*)Kael>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6(C)-Gt(ROSA)26Sor<tm3(HIF1A*)Kael>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6(C)-Gt(ROSA)26Sor<tm3(HIF1A*)Kael>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6(C)-Gt(ROSA)26Sor<tm3(HIF1A*)Kael>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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