Egln1 (EGL nine homolog 1 (C. elegans)) homozygous targeted mutation mice are embryonic lethal at approximately E12.5 due to placental and/or cardiac malformation. No mRNA or protein are detectable in mouse embryonic fibroblasts (MEFs) derived from homozyous mice. This strain may be useful in studies of development.
William G. Kaelin, Dana Farber Cancer Institute
Homozygous targeted mutation mice are embryonic lethal at approximately E12.5 due to placental and/or cardiac malformation. No mRNA or protein are detectable in mouse embryonic fibroblasts (MEFs) derived from homozyous mice. This strain may be useful in studies of development.
An FRT-flanked neomycin resistance cassette was placed in intron 1 of the gene. LoxP sites were introduced just 5' of the cassette and in intron 3. The 129S6/SvEvTac-derived TC1 embryonic stem (ES) cell line was used to create the mutation. A cross with an FVB-actin-flipase strain excised the neomycin cassette and a subsequent FVB EIIA-Cre transgenic line cross removed exons 2 and 3 (which encode almost the entire catalytic domain). The line was backcrossed five times to C57BL/6 by the donating lab to remove the Cre transgene.
|Allele Name||targeted mutation 1.1, William G Kaelin|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Egln1, egl-9 family hypoxia-inducible factor 1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||The floxed exon 2 and 3 of Egln1tm1Kael were removed via cre-mediated recombination by mating with Tg(EIIa-cre)C5379Lmgd mice. Egln1 mRNA and protein are undetectable in MEFs isolated from E12.5 embryos.|
|Mutations Made By|| |
Yoji Andrew Minamishima, Dana-Farber Cancer Institute (DFCI)
When maintained as a live colony, heterozygotes may be bred. Homozygotes are embryonic lethal.
When using the B6.129S6(FVB)-Egln1tm1.1Kael/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009671 in your Materials and Methods section.
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