When these ROSA-DTA mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of diptheria toxin in the cre-expressing cells. These ROSA-DTA mice allow selective ablation in a tissue/cell-specific manner.
Richard M. Locksley, Univ of California San Francisco-HHMI
Homozygotes are viable, fertile and do not display any gross physical or behavioral abnormalities. When these ROSA-DTA mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of diptheria toxin in the cre-expressing cells. These ROSA-DTA mice allow selective ablation in a tissue/cell-specific manner.
Of note, ROSA-DTA mice are also available on a BALB/c congenic background (see Stock No. 009670).
A flox-neo/STOP-flox-diptheria toxin subunit alpha gene cassette was knocked into intron 1 of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for 10 generations by the donating laboratory.
|Expressed Gene||Dta, Diphtheria toxin A chain,|
|Site of Expression|
|Allele Name||targeted mutation 1, Richard M Locksley|
|Allele Type||Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, No functional change)|
|Allele Synonym(s)||targeted mutation 1, Richard M Locksley; Gt(ROSA)26Sortm1(DTA)Lky|
|Gene Symbol and Name||Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano|
|Gene Synonym(s)||beta geo; ROSA26; R26; Gtrgeo26; Gtrosa26; Gtrgeo26; Gtrosa26; AV258896; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; SETD5-AS1; Thumpd3as1|
|Expressed Gene||Dta, Diphtheria toxin A chain,|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A floxed neo/STOP cassette and DTA (diphtheria toxin alpha chain) gene were inserted into the locus. Expression of DTA is only achieved following cre-mediated removal of the floxed neo/STOP cassette.|
|Mutations Made By|| |
Richard Locksley, Univ of California San Francisco-HHMI
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the ROSA-DTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #009669 in your Materials and Methods section.
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