When these ROSA-DTA mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of diptheria toxin in the cre-expressing cells. These ROSA-DTA mice allow selective ablation in a tissue/cell-specific manner.
Richard M. Locksley, Univ of California San Francisco-HHMI
Genetic Background | Generation |
---|---|
N11+N2F4
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, No functional change) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Homozygotes are viable, fertile and do not display any gross physical or behavioral abnormalities. When these ROSA-DTA mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of diptheria toxin in the cre-expressing cells. These ROSA-DTA mice allow selective ablation in a tissue/cell-specific manner.
Of note, ROSA-DTA mice are also available on a BALB/c congenic background (see Stock No. 009670).
A flox-neo/STOP-flox-diptheria toxin subunit alpha gene cassette was knocked into intron 1 of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for 10 generations by the donating laboratory.
Expressed Gene | Dta, Diphtheria toxin A chain, |
---|---|
Site of Expression |
Allele Name | targeted mutation 1, Richard M Locksley |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, No functional change) |
Allele Synonym(s) | R-DTA; ROSA-DTA |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | Dta, Diphtheria toxin A chain, |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 6 |
Molecular Note | A floxed neo/STOP cassette and DTA (diphtheria toxin alpha chain) gene were inserted into the locus. Expression of DTA is only achieved following cre-mediated removal of the floxed neo/STOP cassette. |
Mutations Made By | Richard Locksley, Univ of California San Francisco-HHMI |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the ROSA-DTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #009669 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1(DTA)Lky> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.