B6.129P2-Gt(ROSA)26Sor^tm1(DTA)Lky/J

Stock number: 009669
Product name: ROSA-DTA
Research areas: Research Tools

Description

When these ROSA-DTA mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of diptheria toxin in the cre-expressing cells. These ROSA-DTA mice allow selective ablation in a tissue/cell-specific manner.

Detailed description

Homozygotes are viable, fertile and do not display any gross physical or behavioral abnormalities. When these ROSA-DTA mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of diptheria toxin in the cre-expressing cells. These ROSA-DTA mice allow selective ablation in a tissue/cell-specific manner.

Of note, ROSA-DTA mice are also available on a BALB/c congenic background (see Stock No. 009670).

 

Development

A flox-neo/STOP-flox-diptheria toxin subunit alpha gene cassette was knocked into intron 1 of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for 10 generations by the donating laboratory.

Allele

Symbol: Gt(ROSA)26Sor^tm1(DTA)Lky
Name: targeted mutation 1, Richard M Locksley
MGI accession ID: MGI:3829362
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Inserted expressed sequence; No functional change
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Expressed genes: Dta,Diphtheria toxin A chain,
Molecular note: A floxed neo/STOP cassette and DTA (diphtheria toxin alpha chain) gene were inserted into the locus. Expression of DTA is only achieved following cre-mediated removal of the floxed neo/STOP cassette.