B6N.129(FVB)-Ppargc1a^tm2.1Brsp/J

Stock number: 009666
Product name: Pgc-1α^fl
Research areas: Neurobiology Research, Research Tools

Description

These Ppargc1a (peroxisome proliferative activated receptor, gamma, coactivator 1 alpha) conditional (floxed) mutant mice may be useful in generating conditional mutations for studying hepatic heme biosynthesis and porphyrias as well as neuromuscular junction physiology and muscular dystrophy.

Detailed description

These mice possess loxP sites on either side of exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-5 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase specifically in the liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatic heme biosynthesis and porphyrias.

When bred to a strain expressing Cre recombinase specifically in skeletal muscle, this mutant mouse strain may be useful in studies of neuromuscular junction physiology and muscular dystrophy.

Development

A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 5 of the targeted gene, and another loxP site was inserted upstream of exon 3. This construct was electroporated into "Sv129" derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then crossed to transgenic mice expressing Cre recombinase under the control of the adenovirus EIIa promoter (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J) to remove the neo selection cassette. Mice that retained the loxP site flanked exons 3-5 were then bred to C57BL/6N mice for 7 generations. The EIIa-cre transgene has been removed. Upon arrival at The Jackson Laboratory the mice were crossed with C57BL/6NJ once to establish the colony.

Allele

Symbol: Ppargc1a^tm2.1Brsp
Name: targeted mutation 2.1, Bruce M Spiegelman
MGI accession ID: MGI:5576884
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ppargc1a
Marker name: peroxisome proliferative activated receptor, gamma, coactivator 1 alpha
Chromosome: 5
Strain of origin: 129
Molecular note: Exons 3-5 were flanked by loxP sites. A neo with a third loxP site was located downstream of the floxed exons. Cre-mediated recombination removed the selection cassette.