Mice hemizygous for the BAC LRRK2 transgene "overexpress" wildtype human leucine-rich repeat kinase 2 (LRRK2) gene directed by the human LRRK2 promoter/enhancer regions on the BAC transgene. These BAC LRRK2 mice (also called WT-OX mice) are a control strain for the BAC LRRK2R1441G Parkinson's disease strain (Stock No. 009604).
Chenjian Li, Mount Sinai School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for the BAC LRRK2 transgene are viable and fertile, with expression of a wild-type human leucine-rich repeat kinase 2 (LRRK2) gene directed by the human LRRK2 promoter/enhancer regions on the BAC transgene. These BAC LRRK2 mice (also called WT-OX mice) "overexpress" the wildtype human LRRK2 protein in cortex, cerebellum, striatum and ventral midbrain at an approximately five- to ten-fold greater level than endogenous mouse Lrrk2, and are a control strain for the BAC LRRK2R1441G Parkinson's disease strain (Stock No. 009604). Contrary to the hypokinetic motor deficit of the BAC LRRK2R1441G Parkinson's disease mice, WT-OX mice exhibit slightly increased motor activities compared to nontransgenic wild-type controls.
A human bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 188 kb BAC containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene with 29 kb upstream of the LRRK2 start codon and 42 kb downstream of the LRRK2 stop codon. Bioinformatics analysis predicted that no other genes are present in the BAC. This BAC was microinjected into fertilized FVB/NTac zygotes. Founder BAC LRRK2 mice (also called WT-OX mice) were established and then subsequently maintained by breeding transgenic mice with FVB/N inbred mice for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Expressed Gene | LRRK2, leucine rich repeat kinase 2, human |
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Site of Expression |
Allele Name | transgene insertion 1, Chenjian Li |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | BAC WT LRRK2; TG-WT-OX; WT-OX |
Gene Symbol and Name | Tg(LRRK2)1Cjli, transgene insertion 1, Chenjian Li |
Gene Synonym(s) | |
Promoter | LRRK2, leucine rich repeat kinase 2, human |
Expressed Gene | LRRK2, leucine rich repeat kinase 2, human |
Strain of Origin | FVB/NTac |
Chromosome | UN |
Molecular Note | The human bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 188 kb BAC containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene with 29 kb upstream of the LRRK2 start codon and 42 kb downstream of the LRRK2 stop codon. Bioinformatics analysis predicted that no other genes are present in the BAC. This BAC was microinjected into fertilized FVB/NTac zygotes. |
Mutations Made By | Chenjian Li, Mount Sinai School of Medicine |
When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to FVB/NJ inbred mice (Stock No. 001800).
When using the WT-OX mouse strain in a publication, please cite the originating article(s) and include JAX stock #009610 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(LRRK2)1Cjli/ |
Frozen Mouse Embryo | FVB/N-Tg(LRRK2)1Cjli/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(LRRK2)1Cjli/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(LRRK2)1Cjli/J | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(LRRK2)1Cjli/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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