FVB/N-Tg(LRRK2)1Cjli/J

Stock number: 009610
Product name: WT-OX
Research areas: Neurobiology Research, Research Tools

Description

Mice hemizygous for the BAC LRRK2 transgene "overexpress" wildtype human leucine-rich repeat kinase 2 (LRRK2) gene directed by the human LRRK2 promoter/enhancer regions on the BAC transgene. These BAC LRRK2 mice (also called WT-OX mice) are a control strain for the BAC LRRK2^R1441G Parkinson's disease strain (Stock No. 009604).

Detailed description

Mice hemizygous for the BAC LRRK2 transgene are viable and fertile, with expression of a wild-type human leucine-rich repeat kinase 2 (LRRK2) gene directed by the human LRRK2 promoter/enhancer regions on the BAC transgene. These BAC LRRK2 mice (also called WT-OX mice) "overexpress" the wildtype human LRRK2 protein in cortex, cerebellum, striatum and ventral midbrain at an approximately five- to ten-fold greater level than endogenous mouse Lrrk2, and are a control strain for the BAC LRRK2^R1441G Parkinson's disease strain (Stock No. 009604). Contrary to the hypokinetic motor deficit of the BAC LRRK2^R1441G Parkinson's disease mice, WT-OX mice exhibit slightly increased motor activities compared to nontransgenic wild-type controls.

Development

A human bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 188 kb BAC containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene with 29 kb upstream of the LRRK2 start codon and 42 kb downstream of the LRRK2 stop codon. Bioinformatics analysis predicted that no other genes are present in the BAC. This BAC was microinjected into fertilized FVB/NTac zygotes. Founder BAC LRRK2 mice (also called WT-OX mice) were established and then subsequently maintained by breeding transgenic mice with FVB/N inbred mice for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.

Allele

Symbol: Tg(LRRK2)1Cjli
Name: transgene insertion 1, Chenjian Li
MGI accession ID: MGI:3847810
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(LRRK2)1Cjli
Marker name: transgene insertion 1, Chenjian Li
Chromosome: UN
Strain of origin: FVB/NTac
Expressed genes: LRRK2,leucine rich repeat kinase 2,human
Molecular note: The human bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 188 kb BAC containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene with 29 kb upstream of the LRRK2 start codon and 42 kb downstream of the LRRK2 stop codon. Bioinformatics analysis predicted that no other genes are present in the BAC. This BAC was microinjected into fertilized FVB/NTac zygotes.