STOCK Hprt^{tm32(Ple112-EGFP)Ems}/Mmjax

Stock number: 009594
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple112-EGFP transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human HCRT gene direct expression of EGFP.

Allele

Symbol: Hprt^{tm32(Ple112-EGFP)Ems}
Name: targeted mutation 32, Elizabeth M Simpson
MGI accession ID: MGI:4834653
Generation method: Targeted
Attributes: Reporter
Marker symbol: Hprt
Marker name: hypoxanthine guanine phosphoribosyl transferase
Chromosome: X
Strain of origin: (B6.129P2-Hprt^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP is expressed in the hypothalamus.
Molecular note: The Ple112-EGFP transgene (pEMS1418) was designed with the 1295 bp Ple112 minipromoter (HCRT-B; derived from a subsection of the promoter and an upstream putative regulatory element from the human hypocretin (orexin) neuropeptide precursor (HCRT) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

The promoter/regulatory regions of the human hypocretin (HCRT) gene directs expression of enhanced green fluorescent protein (EGFP) to the hypothalamus. Under the higher magnification, EGFP immunoreactivity can be detected in the cell bodies and processes of hypothalamic neurons. Other (lateral) regions of brain did not display any EGFP-positive cells.
General note: Germ line transmission of mutant cell line mEMS1736 has been established.