B6;129S1-Bambi^tm1Jian/J

Stock number: 009389
Research areas: Neurobiology Research, Research Tools

Description

These Bambi^flox mice harbor loxP sites flanking exon 1 of the Bambi (BMP and activin membrane-bound inhibitor, homolog (Xenopus laevis)) locus, and may be useful in generating conditional mutations for studying the role of Bambi in developmental biology and the TGF-beta pathway.

Detailed description

Mice homozygous for this Bambi^flox allele are viable and fertile, with loxP sites flanking exon 1 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous signal peptide deleted in the cre-expressing tissue(s); this is expected to produce a null allele. These mutant mice may be useful in generating conditional mutations for studying the role of Bambi in developmental biology and the TGF-beta pathway.

Development

A targeting vector was designed to insert a loxP site upstream of exon 1, and a second loxP site followed by an frt-flanked PGK-neo cassette just downstream of exon 1 of the targeted gene. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females to establish the colony. Mice heterozygous for the Bambi^flox allele were bred together to generate homozygotes prior to arrival at The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Bambi^tm1Jian
Name: targeted mutation 1, Rulang Jiang
MGI accession ID: MGI:3758816
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Bambi^flox
Marker symbol: Bambi
Marker name: BMP and activin membrane-bound inhibitor
Marker synonyms: 2610003H06Rik; NMA
Chromosome: 18
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: Exon 1 is flanked by loxP sites and a neo cassette was inserted downstream of exon 1.