These Bambiflox mice harbor loxP sites flanking exon 1 of the Bambi (BMP and activin membrane-bound inhibitor, homolog (Xenopus laevis)) locus, and may be useful in generating conditional mutations for studying the role of Bambi in developmental biology and the TGF-beta pathway.
Rulang Jiang, University of Rochester
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Bambi | BMP and activin membrane-bound inhibitor |
Mice homozygous for this Bambiflox allele are viable and fertile, with loxP sites flanking exon 1 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous signal peptide deleted in the cre-expressing tissue(s); this is expected to produce a null allele. These mutant mice may be useful in generating conditional mutations for studying the role of Bambi in developmental biology and the TGF-beta pathway.
A targeting vector was designed to insert a loxP site upstream of exon 1, and a second loxP site followed by an frt-flanked PGK-neo cassette just downstream of exon 1 of the targeted gene. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females to establish the colony. Mice heterozygous for the Bambiflox allele were bred together to generate homozygotes prior to arrival at The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Rulang Jiang |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Bambiflox |
Gene Symbol and Name | Bambi, BMP and activin membrane-bound inhibitor |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 18 |
Molecular Note | Exon 1 is flanked by loxP sites and a neo cassette was inserted downstream of exon 1. |
Mutations Made By | Rulang Jiang, University of Rochester |
When maintaining a live colony, homozygous mice may be bred.
When using the B6;129S1-Bambitm1Jian/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009389 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Bambi<tm1Jian> |
Frozen Mouse Embryo | B6;129S1-Bambi<tm1Jian>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S1-Bambi<tm1Jian>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S1-Bambi<tm1Jian>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S1-Bambi<tm1Jian>/J Frozen Embryo | $3373.50 |
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