B6.129S1-Osr2^tm1Jian/J

Stock number: 009386
Research areas: Developmental Biology Research, Internal/Organ Research, Research Tools

Description

These Osr2-lacZ mice harbor a nuclear localized β-galactosidase "knock-in" allele that also abolishes endogenous Osr2 (odd-skipped related 2 (Drosophila)) gene function. These mice may be useful as a lacZ reporter for Osr2 expression or as a knockout model for studying developmental biology (craniofacial, limb, and kidney).

Detailed description

The Osr2-lacZ mutation abolishes endogenous gene function and expresses a β-galactosidase fusion protein (fused in-frame with the N-terminal 15 amino acid residues of the endogenous protein). Under control of the upstream promoter/enhancer elements, lacZ expression is directed to the same tissues as the wild-type gene (for example, β-galactosidase expression during embryogenesis is detected by E9.5 in the mesonephric vesicles). Homozygous mice die shortly after birth with open eyelids, bilateral cleft of the secondary palate, and thickened tympanic rings. Heterozygotes are viable and fertile. These Osr2-lacZ mice may be useful as a lacZ reporter for Osr2 expression or as a knockout model for studying developmental biology (craniofacial, limb, and kidney).

Development

A targeting vector was designed to replace exons 2-4 of the Osr2 locus with the initial 15 codons of the Osr2 open reading frame fused in-frame with a nuclear localized β-galactosidase (lacZ) and loxP-flanked PGK-neo cassette. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females to establish the colony. Mice were then backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony. The Y chromosome may not have been fixed to the genetic background during the backcross.

Allele

Symbol: Osr2^tm1Jian
Name: targeted mutation 1, Rulang Jiang
MGI accession ID: MGI:3033240
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Osr2
Marker name: odd-skipped related 2
Chromosome: 15
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: LacZ expression mimics that of the wild-type targeted gene. During embryogenesis, it is detected by E9.5 in the mesonephric vesicles.
Molecular note: A 2.6 kb segment containing Exons 2, 3 and art of 4 was replaced with a lacZ and neomycin resistance cassette. This includes most of the coding region.