These Osr2-lacZ mice harbor a nuclear localized β-galactosidase "knock-in" allele that also abolishes endogenous Osr2 (odd-skipped related 2 (Drosophila)) gene function. These mice may be useful as a lacZ reporter for Osr2 expression or as a knockout model for studying developmental biology (craniofacial, limb, and kidney).
Rulang Jiang, University of Rochester
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Osr2 | odd-skipped related 2 |
The Osr2-lacZ mutation abolishes endogenous gene function and expresses a β-galactosidase fusion protein (fused in-frame with the N-terminal 15 amino acid residues of the endogenous protein). Under control of the upstream promoter/enhancer elements, lacZ expression is directed to the same tissues as the wild-type gene (for example, β-galactosidase expression during embryogenesis is detected by E9.5 in the mesonephric vesicles). Homozygous mice die shortly after birth with open eyelids, bilateral cleft of the secondary palate, and thickened tympanic rings. Heterozygotes are viable and fertile. These Osr2-lacZ mice may be useful as a lacZ reporter for Osr2 expression or as a knockout model for studying developmental biology (craniofacial, limb, and kidney).
A targeting vector was designed to replace exons 2-4 of the Osr2 locus with the initial 15 codons of the Osr2 open reading frame fused in-frame with a nuclear localized β-galactosidase (lacZ) and loxP-flanked PGK-neo cassette. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females to establish the colony. Mice were then backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony. The Y chromosome may not have been fixed to the genetic background during the backcross.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | LacZ expression mimics that of the wild-type targeted gene. During embryogenesis, it is detected by E9.5 in the mesonephric vesicles. |
Allele Name | targeted mutation 1, Rulang Jiang |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Osr2, odd-skipped related 2 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | LacZ expression mimics that of the wild-type targeted gene. During embryogenesis, it is detected by E9.5 in the mesonephric vesicles. |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 15 |
Molecular Note | A 2.6 kb segment containing Exons 2, 3 and art of 4 was replaced with a lacZ and neomycin resistance cassette. This includes most of the coding region. |
Mutations Made By | Rulang Jiang, University of Rochester |
When maintaining a live colony, heterozygous mice may be bred to wildtype siblings or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes die shortly after birth.
When using the B6.129S1-Osr2tm1Jian/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009386 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Osr2<tm1Jian> |
Frozen Mouse Embryo | B6.129S1-Osr2<tm1Jian>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Osr2<tm1Jian>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Osr2<tm1Jian>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Osr2<tm1Jian>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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