These Irf4 (interferon regulatory factor 4) conditional (floxed) mutant mice may be useful in generating conditional mutations for studying plasma cell development, immunoglobulin class switch recombination and autoimmune lymphoproliferative disease. Cre-mediated recombination also results in expression of GFP (Green Fluorescent Protein) which can be used to trace Irf4 ablation.
Ulf Klein, University of Leeds
Genetic Background | Generation |
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N9+N3F3
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Irf4 | interferon regulatory factor 4 |
These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1 and 2 deleted and GFP expression in the cre-expressing tissue(s). When crossed to mice that express Flp recombinase, the entire targeting construct, including exons 1 and 2, are deleted in the FLP expressing tissues.
When bred to a strain expressing Cre recombinase in B lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of plasma cell development and immunoglobulin class switch recombination.
When bred to a strain expressing Cre recombinase in T regulatory cells, this mutant mouse strain may be useful in studies of autoimmune lymphoproliferative disease.
A loxP site flanked targeting vector containing a PGKneo cassette was utilized in the construction of this mutant. Sequence encoding a promoterless eGFP minigene, a loxP site and the PGKneo cassette was inserted upstream of exon 1 of the targeted gene, and another loxP site followed by the PGK promoter was inserted downstream of exon 2. The entire targeted region was flanked by FRT sites. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric male animals were crossed to C57BL/6 females, and were then backcrossed to C57BL/6 for 8 generations.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Cre-mediated recombination results in expression of GFP which may be used to trace tissue/cell-specific Irf4 ablation. |
Allele Name | targeted mutation 1, Riccardo Dalla-Favera |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Irf4fl |
Gene Symbol and Name | Irf4, interferon regulatory factor 4 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Cre-mediated recombination results in expression of GFP which may be used to trace tissue/cell-specific Irf4 ablation. |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 13 |
Molecular Note | A targeting construct was designed to insert an frt-EGFP-loxP sequence upstream of exon 1 and a loxP-PGK-frt downstream of exon 2 such that cre mediated excision would remove the exons while allowing EGFP expression and, alternatively, FLP-mediated excision would remove both the exons and the EGFP. |
Mutations Made By | Ulf Klein, University of Leeds |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Irf4fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #009380 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Irf4<tm1Rdf> |
Frozen Mouse Embryo | B6.129S1-Irf4<tm1Rdf>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Irf4<tm1Rdf>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Irf4<tm1Rdf>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Irf4<tm1Rdf>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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