These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1 and 2 deleted and GFP expression in the cre-expressing tissue(s). When crossed to mice that express Flp recombinase, the entire targeting construct, including exons 1 and 2, are deleted in the FLP expressing tissues.
When bred to a strain expressing Cre recombinase in B lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of plasma cell development and immunoglobulin class switch recombination.
When bred to a strain expressing Cre recombinase in T regulatory cells, this mutant mouse strain may be useful in studies of autoimmune lymphoproliferative disease.
