B6.129S1-Bptf^tm1.1Cwu/J

Stock number: 009367
Research areas: Research Tools

Description

These Bptf (bromodomain PHD finger transcription factor) mutant mice carry a loxP site flanked (floxed) exon 2 and may be useful in generating conditional mutations for studying chromatin remodeling during development.

Detailed description

These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying chromatin remodeling during development.

Development

A loxP site flanked targeting vector containing a neo cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺ derived CJ7 embryonic stem (ES) cells. Correcting targeted ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exon 2 were injected in recipient blastocysts. The resulting chimeric mice were crossed to a Flp recombinase expressing strain (N10 on the C57BL/6NCr background) to delete the Neo cassette while retaining loxP sites flanking exon 2. The mice were backcrossed to wildtype C57BL/6NCr mice for 12 generations and no longer carry the Flp expressing transgene. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6NJ once.

Allele

Symbol: Bptf^tm1.1Cwu
Name: targeted mutation 1.1, Carl Wu
MGI accession ID: MGI:3841297
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Bptf^floxed; Bptf^tm1Cwu
Marker symbol: Bptf
Marker name: bromodomain PHD finger transcription factor
Marker synonyms: 9430093H17Rik; FAC1; Falz; MGC:54978; NEDDFL; NURF301
Chromosome: 11
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: A loxP site was inserted upstream of exon 2, and a frt-flanked neomycin cassette and a downstream loxP was inserted downstream of exon 2. Flpe expression removed the neomycin cassette leaving exon 2 floxed.
General note: Phenotypic Similarity to Human Syndrome in Orthologous Human Gene: NEURODEVELOPMENTAL DISORDER WITH DYSMORPHIC FACIES AND DISTAL LIMB ANOMALIES; NEDDFL (OMIM:617755) in conditional knockout mice homozygous for Bptf^tm1.1Cwu and heterozygous for Emx1^tm1(cre)Krj (J:334252).