Homozygous Lats1 (large tumor suppressor) animals show hyperplastic changes in the pituitary and decreased serum hormone levels. Reproductive hormone defects are reminiscent of isolated luteinizing hormone (LH)-hypogonadotropic hypogonadism and corpus luteum insufficiency in humans. Homozygous mice develop soft tissue sarcomas and ovarian stromal cell tumors and are highly sensitive to carcinogenic treatments.
Tian Xu, Yale University School of Medicine, HHMI
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Lats1 | large tumor suppressor |
Homozygous animals exhibit a lack of mammary gland development, infertility and growth retardation. Accompanying these defects are hyperplastic changes in the pituitary and decreased serum hormone levels. The reproductive hormone defects of homozygotes are reminiscent of isolated luteinizing hormone (LH)-hypogonadotropic hypogonadism and corpus luteum insufficiency in humans. Furthermore, Homozygous mice develop soft tissue sarcomas (some by 4-10 months of age) and ovarian stromal cell tumors (by 3 months of age) and are highly sensitive to carcinogenic treatments. Data demonstrates a role for this gene in mammalian tumorigenesis and specific endocrine dysfunction.
A neomycin resistance cassette replaces sequences that correspond to human gene amino acids 756-1130. The targeting vector was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
Allele Name | targeted mutation 1, Tian Xu |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Lats1- |
Gene Symbol and Name | Lats1, large tumor suppressor |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 10 |
Molecular Note | A neomycin resistance cassette replaced sequence encoding amino acids 756 - 1130 of the protein. Immunoprecipitation and Western blots of embryonic fibroblasts lysates from homozygous mutant mice showed no detectable protein for the targeted gene. |
Mutations Made By | Tian Xu, Yale University School of Medicine, HHMI |
When maintained as a live colony, heterozygotes may be bred. Recovery of homozygotes is reduced (approximately 5% from heterozygote x heterozygote crosses) as is postnatal survival. Homozygous males display decreased fertility and females display severe fertility defects.
When using the B6;129S2-Lats1tm1Tx/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009358 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Lats1<tm1Tx> |
Frozen Mouse Embryo | B6;129S2-Lats1<tm1Tx>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Lats1<tm1Tx>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Lats1<tm1Tx>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S2-Lats1<tm1Tx>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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