ISS/IbgTejJ

Stock number: 009325
Research areas: Neurobiology Research, Research Tools

Description

The ISS (Inbred Short Sleep) strain is the product of a selection for long duration (sleep time) of loss-of-righting response following high dose ip administration of ethanol. This strain and ISS are the progenitors used to generate the ILSXISS recombinant inbred strain set used in QTL mapping analysis.

Detailed description

The ISS (Inbred Short Sleep) strain is the product of a selection for long duration (sleep time) of loss-of-righting response following high dose (3.3g/kg) intraperitoneal administration of ethanol. During the selection process, the SS mice developed insensitivity to alcohol requiring a dose increase to 4.1 g/kg. At the 18th generation of inbreeding SS (short-sleep) mice exhibited a mean sleep time of 10 minutes. This strain and ISS are the progenitors used to generate the ILSXISS recombinant inbred strain set used in QTL mapping analysis.

Development

The ISS and SS strains were developed from a heterogenous stock derived from an 8 way cross (A, AKR, BALB/c, C3H/2, C57BL, DBA/2, IS/Bi and RIII) created at the Cancer Research Genetics Laboratory at the University of California, Berkley. ISS is the product of inbreeding SS, a line selected for short duration (sleep time) of loss-of-righting response following high dose (3.3g/kg) intraperitoneal administration of ethanol. During the selection process, the SS mice developed insensitivity to alcohol requiring a dose increase to 4.1 g/kg. At the 18th generation of inbreeding SS (short-sleep) mice exhibited a mean sleep time of 10 minutes. The name SS became ISS (inbred short-sleep) in the 20th generation of inbreeding.

This strain and ILS/IbgTej (inbred long-sleep) were used to generate the ILSXISS recombinant inbred set donated to The Jackson Laboratory Repository in 2009 by Dr. Thomas E. Johnson and Dr. Jerry Stitzel at the University of Colorado, Boulder. An earlier RI set, LSXSS, was created using the LS and SS mice - the Repository does not maintain this set.