B6;FVB-Tg(Cnp-EGFP/Rpl10a)JD368Htz/J

Stock number: 009159
Product name: CNP1 bacTRAP
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice express the EGFP-L10a fusion gene, under the control of the mouse Cnp promoter. Green fluorescent protein is detected in mature oligodendrocytes.

Detailed description

These BAC TRAP transgenic mice express the EGFP-L10a fusion gene, EGFP/Rpl10a, under the control of the mouse Cnp (2',3'-cyclic nucleotide 3' phosphodiesterase) promoter. The transgene expression pattern corresponds to endogenous Cnp expression. Green fluorescent protein is detected in mature oligodendrocytes. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Transgenic male mice are infertile. Attempts at cryopreserving sperm from male transgenic mice have failed. This mutant mouse strain may be useful in affinity purification of polysomal mRNAs (translating ribosome affinity purification or TRAP) from mature oligodendrocytes and tracking mature oligodendrocytes by fluorescence.

Development

The mouse bacterial artificial chromosome (BAC) RP23-78L12 containing the entire Cnp (2',3'-cyclic nucleotide 3' phosphodiesterase) gene, was modified by insertion of the EGFP-L10a fusion gene, EGFP/Rpl10a, into the translation start site. The resulting BAC transgene was microinjected into FVB donor eggs. Founder line JD368 was subsequently established. The mice were then crossed to C57BL/6J for four generations before arriving at The Jackson Laboratory. The mice were then crossed to C57BL/6J once to establish the colony. A 32 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory in February 2011 revealed that this strain is segregating at 3-6 markers across chromosomes 4, 8, 11, 13, 15 and 19.

Allele

Symbol: Tg(Cnp-EGFP/Rpl10a)JD368Htz
Name: transgene insertion JD368, Nathaniel Heintz
MGI accession ID: MGI:3850676
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Cnp-EGFP/Rpl10a)JD368Htz
Marker name: transgene insertion JD368, Nathaniel Heintz
Chromosome: UN
Strain of origin: FVB
Expressed genes: GFP,Green Fluorescent Protein,; Rpl10a,ribosomal protein L10A,mouse, laboratory
Site of expression: mature oligodendrocytes
Molecular note: The mouse bacterial artificial chromosome (BAC) RP23-78L12 containing the entire Cnp (2',3'-cyclic nucleotide 3' phosphodiesterase) gene, was modified by insertion of the EGFP-L10a fusion gene, EGFP/Rpl10a, into the translation start site. The resulting BAC transgene was microinjected into FVB donor eggs. Founder line JD368 was subsequently established.