These transgenic mice express the EGFP-L10a fusion gene, under the control of the mouse Cnp promoter. Green fluorescent protein is detected in mature oligodendrocytes.
Nathaniel Heintz, The Rockefeller University
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
These BAC TRAP transgenic mice express the EGFP-L10a fusion gene, EGFP/Rpl10a, under the control of the mouse Cnp (2',3'-cyclic nucleotide 3' phosphodiesterase) promoter. The transgene expression pattern corresponds to endogenous Cnp expression. Green fluorescent protein is detected in mature oligodendrocytes. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Transgenic male mice are infertile. Attempts at cryopreserving sperm from male transgenic mice have failed. This mutant mouse strain may be useful in affinity purification of polysomal mRNAs (translating ribosome affinity purification or TRAP) from mature oligodendrocytes and tracking mature oligodendrocytes by fluorescence.
The mouse bacterial artificial chromosome (BAC) RP23-78L12 containing the entire Cnp (2',3'-cyclic nucleotide 3' phosphodiesterase) gene, was modified by insertion of the EGFP-L10a fusion gene, EGFP/Rpl10a, into the translation start site. The resulting BAC transgene was microinjected into FVB donor eggs. Founder line JD368 was subsequently established. The mice were then crossed to C57BL/6J for four generations before arriving at The Jackson Laboratory. The mice were then crossed to C57BL/6J once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory in February 2011 revealed that this strain is segregating at 3-6 markers across chromosomes 4, 8, 11, 13, 15 and 19.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Expressed Gene | Rpl10a, ribosomal protein L10A, mouse, laboratory |
Site of Expression | mature oligodendrocytes |
Allele Name | transgene insertion JD368, Nathaniel Heintz |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | CNP1 bacTRAP |
Gene Symbol and Name | Tg(Cnp-EGFP/Rpl10a)JD368Htz, transgene insertion JD368, Nathaniel Heintz |
Gene Synonym(s) | |
Promoter | Cnp, 2',3'-cyclic nucleotide 3' phosphodiesterase, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Rpl10a, ribosomal protein L10A, mouse, laboratory |
Site of Expression | mature oligodendrocytes |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | The mouse bacterial artificial chromosome (BAC) RP23-78L12 containing the entire Cnp (2',3'-cyclic nucleotide 3' phosphodiesterase) gene, was modified by insertion of the EGFP-L10a fusion gene, EGFP/Rpl10a, into the translation start site. The resulting BAC transgene was microinjected into FVB donor eggs. Founder line JD368 was subsequently established. |
Mutations Made By | Joseph Dougherty, Washington University |
When maintaining a live colony, these mice can be bred as hemizygotes. Male transgenic mice are infertile.
When using the CNP1 bacTRAP mouse strain in a publication, please cite the originating article(s) and include JAX stock #009159 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or wildtype females and Hemizygous or Non carrier males for Tg(Cnp-EGFP/Rpl10a)JD368Htz |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.