Myd88 KO mice have hematopoietic system defects, as well as signaling and apoptotic defects. They have increased susceptibility to bacterial and viral infection due to immune system abnormalities.
IMR Colony, The Jackson Laboratory
The Myd88-deficient allele encodes a deletion of exon 3 of the myeloid differentiation primary response gene 88 locus. Myd88-deficiency is associated with a number of immune system abnormalities, as well as hematopoietic system and apoptotic abnormalities.
Homozygous mice are viable and fertile. The Myd88-deficient allele encodes a deletion of exon 3 of the myeloid differentiation primary response gene 88 gene. MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immuneresponse. MyD88 signaling is required to limit bacterial burden and prolong survival during infection. Myd88-deficiency is associated with a number of immune system abnormalities, as well as hematopoietic system, molecular signaling, and apoptotic abnormalities. These mice have increased susceptibility to bacterial and viral infection and should be maintained in a specific pathogen free environment.
Myd88fl mice, harboring loxP sites on either side of exon 3 of the Myd88 gene, arrived at The Jackson Laboratory congenic on the C57BL/6J genetic background (see Stock No. 008888). Upon arrival, some of these Myd88fl mice were bred with C57BL/6-Tg(Zp3-Cre)93Knw/J mice (Stock No. 003651) for germline removal of loxP-flanked exon 3. Offspring with the floxed exon 3 removed were identified and then selectively bred to C57BL/6J inbred mice to remove the Cre transgene. The resulting mice, heterozygous for the Myd88-deficient allele, were subsequently bred together, to wildtype siblings, or to C57BL/6J inbred mice to establish this strain (as Stock No. 009088).
|Allele Name||targeted mutation 1.1, Anthony L DeFranco|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Myd88, myeloid differentiation primary response gene 88|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Mice carrying the Myd88tm1Defr allele were crossed with Tg(Zp3-cre)93Knw mice to remove exon 3.|
|Mutations Made By|| |
Anthony DeFranco, UCSF
When maintaining a live colony, homozygous mice may be bred together. These mice have increased susceptibility to bacterial and viral infection and should be maintained in a specific pathogen free environment.
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