Mice homozygous for the Ret- allele harbor a knockout of the ret proto-oncogene locus and may be useful in studying kidney/urinary and peripheral nervous system development, as well as abnormal enteric neural crest cell migration and Hirschsprung's Disease.
Frank Costantini, Columbia University Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ret | ret proto-oncogene |
The Ret- allele (also called ret-k-, ret-k minus, or c-ret-) disrupts the region of the ret proto-oncogene (Ret; also called ret-k or c-ret) locus harboring the invariant lysine codon required for Ret kinase activity. Homozygous mice die around 16-24 hours after birth, exhibiting abnormalities in kidney/urinary (renal agenesis/hypodysplasia) and peripheral nervous system development (including sympathetic, parasympathetic, and enteric ganglia), as well as abnormal enteric neural crest cell migration. Because homozygous mice lack enteric ganglia from the hindgut, these mice are also a model of Hirschsprung's Disease.
A targeting vector was designed to replace an 0.8 kb region of the ret proto-oncogene (Ret; also called ret-k or c-ret) locus, including the codon for an invariant lysine required for Ret kinase activity, with a neomycin resistance cassette. The construct was electroporated into 129S/SvEv-Gpi1c derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129/SvEv mice to establish the colony. Heterozygous mice were bred with wildtype 129/SvEv (and/or 129S6/SvEvTac) mice for many generations prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred together 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Frank Costantini |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | c-ret-; Ret-; ret-k- |
Gene Symbol and Name | Ret, ret proto-oncogene |
Gene Synonym(s) | |
Strain of Origin | 129S/SvEv-Gpi1c |
Chromosome | 6 |
Molecular Note | A neomycin resistance cassette replaced 0.8 kb of sequence, including the codon for an invariant lysine required for kinase activity. Northern blot analysis did not detect normal transcripts in brains of homozygous mice, but did detect several transcripts with sizes consistent with predicted products of the mutant allele. |
Mutations Made By | Frank Costantini, Columbia University Medical Center |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings or to 129S1/SvImJ inbred mice (Stock No. 002448). Homozygous mice die around one day after birth.
When using the 129S/Sv-Rettm1Cos/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009085 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ret<tm1Cos> |
Frozen Mouse Embryo | 129S/Sv-Ret<tm1Cos>/J | $2595.00 |
Frozen Mouse Embryo | 129S/Sv-Ret<tm1Cos>/J | $2595.00 |
Frozen Mouse Embryo | 129S/Sv-Ret<tm1Cos>/J | $3373.50 |
Frozen Mouse Embryo | 129S/Sv-Ret<tm1Cos>/J | $3373.50 |
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