Homozygous Rcan1 (regulator of calcineurin 1) targeted mice demonstrate an approximate 10% reduction in body weight as adults and diminished fertility as they age. Mice show a significant reduction of NFAT (nuclear factor of activated T cells) transcriptional activity that is sustained in mouse embryonic fibroblasts (MEFs) for eight hours after thapsigargin stimulation to increase intracellular Ca2+. This evidence suggests a facilitator role for the gene in efficient calcineurin-NFAT coupling. Mice are also resistant to induced cardiac hypertrophy.
Jeffery D. Molkentin, Cincinnati Children's Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Rcan1 | regulator of calcineurin 1 |
Homozygotes demonstrate an approximate 10% reduction in body weight as adults and diminished fertility as they age. Mice show a significant reduction of NFAT (nuclear factor of activated T cells) transcriptional activity that is sustained in mouse embryonic fibroblasts (MEFs) for eight hours after thapsigargin stimulation to increase intracellular Ca2+. This evidence suggests a facilitator role for the gene in efficient calcineurin-NFAT coupling. Mice are also resistant to induced cardiac hypertrophy.
CD4+ T cells of compound Rcan1/Rcan2 (Mcip1/Mcip2) targeted mutants show enhanced apoptosis. Slow/oxidative fiber-type switching is impaired in their skeletal muscle after exercise. A neurological phenotype involving increased locomotor activity and impaired working memory is also seen in the compound mutants. For more information on the Rcan2 mutation, please see Stock No. 009073).
Exons 5 ad 6 were replaced with a neomycin resistance cassette in a 129-derived embryonic stem (ES) cell line. Chimeric mice were crossed to C57BL/6 and maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
Allele Name | targeted mutation 1, Jeffery D Molkentin |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | mcip1- |
Gene Symbol and Name | Rcan1, regulator of calcineurin 1 |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 16 |
Molecular Note | Exons 5 and 6 were replaced with a neomycin resistance gene via homologous recombination. Wesetern blot confirmed absence of protein in mutant heart, skeletal muscle and brain. |
Mutations Made By | Jeffery Molkentin, Cincinnati Children's Hospital |
When maintained as a live colony, homozygotes or heterozygotes may be bred. Fertility of the homozygotes is diminished as they age.
When using the B6;129-Rcan1tm1Jmol/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009072 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Rcan1<tm1Jmol> |
Frozen Mouse Embryo | B6;129-Rcan1<tm1Jmol>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Rcan1<tm1Jmol>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Rcan1<tm1Jmol>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Rcan1<tm1Jmol>/J Frozen Embryo | $3373.50 |
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