Homozygous Cc2d1a (coiled-coil and C2 domain containing 1A) targeted mutant mice are born at predicted Mendelian ratios but die soon after birth from an inability to breathe. Homozygotes display no physical abnormalities, based on hematoxylin and eosin (H&E) staining. Defects in brain function may explain the phenotype.
Zhijian 'James' Chen, UT Southwestern
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cc2d1a | coiled-coil and C2 domain containing 1A |
Homozygous mutant mice are born at predicted Mendelian ratios but die soon after birth from an inability to breathe. No protein is detected by Western blot of embryonic fibroblast cells. No mRNA is detected by in situ hybridization. Homozygotes display no physical abnormalities, based on hematoxylin and eosin (H&E) staining. Defects in brain function may explain the phenotype.
A targeting vector containing a beta galactosidase gene (lacZ), and a loxP-flanked neomycin resistance cassette was used to replace exons 1-10. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 6 generations.
Allele Name | targeted mutation 1, Zhijian Chen |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | 1AKO-A; Mit5- |
Gene Symbol and Name | Cc2d1a, coiled-coil and C2 domain containing 1A |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 8 |
Molecular Note | A targeting vector containing a beta galactosidase gene (lacZ), followed by a loxP-flanked neomycin resistance cassette was fused in-frame to exon 1. The tergeting vector deleted exons 1-10 (amino acids 3-349) of the gene. Southern and Western blot failed to detect DNA or protein, respectively, in mutants. |
Mutations Made By | Meng Zhao |
When maintained as a live colony, heterozygotes may be bred. Homozygotes die soon after birth.
When using the B6.129S6-Cc2d1atm1Zjc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009057 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Cc2d1a<tm1Zjc> |
Frozen Mouse Embryo | B6.129S6-Cc2d1a<tm1Zjc>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Cc2d1a<tm1Zjc>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Cc2d1a<tm1Zjc>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Cc2d1a<tm1Zjc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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