The Tie2-Cre (Tek-Cre) transgene has the mouse endothelial-specific receptor tyrosine kinase (Tek or Tie2) promoter directing expression of Cre recombinase. These Tek-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in endothelial cells during embryogenesis and adulthood.
Dr. Masashi Yanagisawa, Southwestern Medical School
Genetic Background | Generation |
---|---|
N8+pN2F5
|
Allele Type |
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Transgenic (Recombinase-expressing, Hypomorph) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek (Tie2) promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in vascular endothelial cells. Cre recombinase activity is also detected in reproductive tissues (endometrium, ovary, testis, epididymis), as well as extraembryonic vascular system and yolk sac.
In 2017, it was discovered that 2 copies of the transgene integrated approximately 15 kB into intron 1 of the Sorcs2
gene on Chr 5 (between nucleotides 36382105 to 36382309 GRCm37/mm9
genome build) resulting in a 204 bp deletion. The insertion leads to reduced and variable expression of Sorcs2. Mice homozygous for the transgene exhibit abnormal cochlear stereociliary bundle formation on both inner and outer hair cells along the organ of Corti, decreased body weight and deafness.
Cre cDNA and metallothionein-1 (MT-1) polyA signal sequence was inserted between the mouse Tek (Tie2) promoter and enhancer. The transgenic cassette was injected into B6SJLF1 fertilized oocytes and backcrossed to a C57BL/6 genetic background for at least eight generations by the donating investigator.
In 2017, it was discovered that 2 copies of the transgene integrated approximately 15 kB into intron 1 of the Sorcs2
gene on Chr 5 (between nucleotides 36382105 to 36382309 GRCm37/mm9
genome build) resulting in a 204 bp deletion.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | uniform expression in endothelial cells during embryogenesis and adulthood. |
Allele Name | transgene insertion 1, Masashi Yanagisawa |
---|---|
Allele Type | Transgenic (Recombinase-expressing, Hypomorph) |
Allele Synonym(s) | T2Cre; TekCre; Tie-2 Cre; Tie2Cre; Tie2-Cre; Tie2Cre+ |
Gene Symbol and Name | Tg(Tek-cre)1Ywa, transgene insertion 1, Masashi Yanagisawa |
Gene Synonym(s) | |
Promoter | Tek, TEK receptor tyrosine kinase, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | uniform expression in endothelial cells during embryogenesis and adulthood. |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | 5 |
Molecular Note | This transgene expresses Cre recombinase under the control of a mouse Tek promoter and enhancer. This promoter is active in endothelial cells. Insertion within intron 1 of Sorcs2 results in the 204 bp deletion (36382105 to 36382309 on mouse chromosome 5; GRCm37/mm9 genome build) leading to reduced and variable expression. |
Mutations Made By | Dr. Masashi Yanagisawa, Southwestern Medical School |
When maintained as a live colony, hemizygotes may be bred.
When using the Tie2-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008863 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Tek-cre)1Ywa |
Frozen Mouse Embryo | B6.Cg-Tg(Tek-cre)1Ywa/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Tek-cre)1Ywa/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Tek-cre)1Ywa/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Tek-cre)1Ywa/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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