These transgenic mice express a tamoxifen-inducible cre recombinase from the endogenous promoter/enhancer elements of the elastase 1 locus. When induced, cre activity is observed in Ela1 expressing pancreatic acinar cells; making this strain useful in fate mapping of pancreatic acinar cells.
Doris A Stoffers, University of Pennsylvania
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
These transgenic mice have a tamoxifen-inducible, Cre-mediated recombination system driven by the rat elastase 1, pancreatic (Ela1) promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain (CreERT2). The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. No cre activity is detectable without tamoxifen treatment. When these transgenic mice are bred with mice containing a loxP flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Ela1
expressing cells; making them useful in fate mapping of pancreatic acinar cells.
Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous.
A transgenic construct containing sequence encoding cre/ESR1 (CreERT2) under the control of the rat elastase 1, pancreatic (Ela1) promoter and rabbit Beta-globin intron and polyadenylation cassette, was introduced into B6SJLF1 donor eggs. Founder animals were bred to CD-1 mice.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | When mice are bred with mice containing a loxP flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Ela1 expressing pancreatic acinar cells. |
Allele Name | transgene insertion 1, Doris A Stoffers |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | ElastaseCreERT2; Tg(Ela1-Cre/ESR1)1Stof |
Gene Symbol and Name | Tg(Cela1-cre/ERT2)1Stof, transgene insertion 1, Doris A Stoffers |
Gene Synonym(s) | |
Promoter | Cela1, chymotrypsin-like elastase family, member 1, rat |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | When mice are bred with mice containing a loxP flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Ela1 expressing pancreatic acinar cells. |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | UN |
Molecular Note | A transgenic construct containing the rabbit Beta-globin (RBG) intron, a sequence encoding Cre/ESR1 (Cre recombinase fused to a mutant estrogen receptor ligand-binding domain (ERT2)) under the control of the acinar cell-specific rat elastase 1 (Cela1) promoter, and a rabbit Beta-globin polyadenylation cassette was made and injected into B6SJLF1 oocytes. |
Mutations Made By | Doris Stoffers, University of Pennsylvania |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous.
When using the ElastaseCreERT2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #008861 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Ela1-Cre/ESR1)1Stof |
Frozen Mouse Embryo | STOCK Tg(Cela1-Cre/ERT2)1Stof/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Cela1-Cre/ERT2)1Stof/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Cela1-Cre/ERT2)1Stof/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Cela1-Cre/ERT2)1Stof/J | $3373.50 |
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