B6.129(C)-Smn1^tm1.1Jme/J

Stock number: 008849
Research areas: Developmental Biology Research, Neurobiology Research

Description

Homozygotes of this targeted mutation strain are embryonic lethal. This strain may be useful in studies of Spinal Muscular Atrophy.

Detailed description

Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes have an embryonic lethal phenotype and die at embryonic day 9. Exon 7 of Smn1 was excised via Cre recombination. The Donating Investigator reports that the phenotype of this strain is similar to the phenotype exhibited by mice carrying the Smn1^tm1Hung allele.

Development

B6.129-Smn1^tm1Jme/J (Stock No. 006146) mice were crossed with B6.C-Tg(CMV-cre)1Cgn/J (STOCK No. 6054) mice to delete the floxed exon 7. The resulting mice were maintained as heterozygotes, using a heterozygote X wildtype sibling mating scheme. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Smn1^tm1.1Jme
Name: targeted mutation 1.1, Judith Melki
MGI accession ID: MGI:2384155
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Smn1
Marker name: survival motor neuron 1
Chromosome: 13
Strain of origin: 129
Molecular note: Animals heterozygous for the targeted mutation Smn^tm1Jme were mated to a cre recombinase transgenic line to delete the floxed exon 7. Deletion of exon 7 in mRNA from the resulting animals was confirmed by RT-PCR analysis. Heterozygous exon 7 deletion animals were intercrossed to generate homozygous mutants. Homozygous mutant embryos were detected by PCR genotype analysis at E9.5.