B6(Cg)-Aicda^tm4.1Mnz/J

Stock number: 008826
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These mice carry a threonine to alanine (T140A) phosphorylation site mutation in exon 3 of the activation-induced cytidine deaminase gene. B cells are impaired but not ablated in their ability to undergo class switch recombination and somatic hypermutation.

Detailed description

These mice carry a threonine to alanine (T140A) phosphorylation site mutation in exon 3 of the activation-induced cytidine deaminase gene. Homozygotes are developmentally normal. B cells are impaired but not ablated in their ability to undergo class switch recombination and somatic hypermutation.

Development

Exon 3's threonine 140 was mutated to alanine (bp change is A95G) and a loxP-flanked neomycin resistance cassette was place in intron 2. A B6(Cg)-Tyr^c-2J/J-derived embryonic stem cell line was used to create the mutation. The neomycin cassette was excised with Cre, leaving a single loxP site. A C57BL/6 genetic background was maintained by the donating laboratory.

Allele

Symbol: Aicda^tm4.1Mnz
Name: targeted mutation 4.1, Michel C Nussenzweig
MGI accession ID: MGI:3839323
Generation method: Targeted
Marker symbol: Aicda
Marker name: activation-induced cytidine deaminase
Chromosome: 6
Strain of origin: B6(Cg)-Tyr^c-2J/J
Molecular note: Threonine 140 of Exon 3 was mutated to alanine (by introducing an A95G base pair change) and a loxP-flanked neomycin resistance cassette was placed in intron 2. A B6(Cg)-Tyr^c-2J/J-derived embryonic stem cell line was used to create the mutation. The neomycin cassette was excised with Cre, leaving a single loxP site.