C57BL/6-Aicda^tm3.1Mnz/J

Stock number: 008825
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These mice carry a serine 38 to alanine (S38A) phosphorylation site mutation in exon 2 of the Aicda (activation-induced cytidine deaminase) gene. B cells are impaired but not ablated in their ability to undergo class switch recombination and somatic hypermutation. These mice also have larger germinal centers in their lymph nodes than wildtype animals.

Detailed description

These mice carry a serine 38 to alanine (S38A) phosphorylation site mutation in exon 2 of the activation-induced cytidine deaminase gene. Homozygotes are developmentally normal. B cells are impaired but not ablated in their ability to undergo class switch recombination and somatic hypermutation. These mice also have larger germinal centers in their lymph nodes than wildtype animals.

Development

Exon 2's serine 38 was mutated to alanine and a loxP-flanked neomycin resistance cassette was place in intron 2. A C57BL/6-derived embryonic stem cell line was used to create the mutation. The neomycin cassette was excised with Cre, leaving a single loxP site. A C57BL/6 genetic background was maintained by the donating laboratory.

Allele

Symbol: Aicda^tm3.1Mnz
Name: targeted mutation 3.1, Michel C Nussenzweig
MGI accession ID: MGI:3839329
Generation method: Targeted
Marker symbol: Aicda
Marker name: activation-induced cytidine deaminase
Chromosome: 6
Strain of origin: C57BL/6
Molecular note: Serine 38 of Exon 2 was mutated to alanine and a loxP-flanked neomycin resistance cassette was place in intron 2. A C57BL/6-derived embryonic stem cell line was used to create the mutation. The neomycin cassette was excised with Cre, leaving a single loxP site.