Mouse Strain Datasheet - 008813
Mouse Strain Datasheet - 008813
These compound mutant mice carry a floxed allele of the Trpa1 (transient receptor potential cation channel, subfamily A, member 1) gene in addition to a tamoxifen inducible cre transgene driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer (see Stock No. 004682). Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces cre recombination within the Trpa1 allele, excising the S5/S6 transmembrane domains. Animals not treated with tamoxifen display no observable phenotype.
David P. Corey, Harvard Medical School/HHMI
| Genetic Background | Generation |
|---|---|
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| Allele Type |
|---|
| Transgenic (Inducible, Recombinase-expressing) |
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Targeted (Conditional ready (e.g. floxed), No functional change) | Trpa1 | transient receptor potential cation channel, subfamily A, member 1 |
These compound mutant mice carry a floxed allele of the Trpa1 (transient receptor potential cation channel, subfamily A, member 1) gene in addition to a tamoxifen inducible cre transgene driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer (see Stock No. 004682). Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces cre recombination within the Trpa1 allele, excising the S5/S6 transmembrane domains. Animals not treated with tamoxifen display no observable phenotype.
This compound mutant strain was created by intercrossing a Trpa1 floxed strain with a tamoxifen-inducible cre mutation strain (see Stock No. 004682).
To create the Trpa1 floxed allele, loxP sites were placed on either side of the exons that encode the S5/S6 transmembrane domains. An FRT-flanked self-excising neomycin resistance cassette was initially placed within the floxed allele, and was deleted. The mutation was created with 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells.
This compound mutant strain is on a mixed genetic background that involves C57BL/6, CBA, and 129.
| Expressed Gene | cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene, |
|---|---|
| Site of Expression | tamoxifen-inducible cre; widespread pattern of expression; tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice |
| Site of Expression |
| Allele Name | transgene insertion 5, Andrew P McMahon |
|---|---|
| Allele Type | Transgenic (Inducible, Recombinase-expressing) |
| Allele Synonym(s) | CAG-CreERT2; CAG-CreEr; CAGG-Cre; CAGG-CreERT2; CAGGCre-ER; CAGGCre-ERTM; CAGGCre-ERtm line 5.8; CAGGS-CreER; CAGGS-CreErT; CMV-creERT; CaggCreER; Cre-ER; Cre-ERTM; CreESRT; ER-cre; Tg(CAG-cre/Esr1)5Amc; Tg(cre/Esr1)5Amc; Tg:Cag-Cre/Esr1; TgCreER; TgCAGGCreER; uCreERT |
| Gene Symbol and Name | Tg(CAG-cre/Esr1*)5Amc, transgene insertion 5, Andrew P McMahon |
| Gene Synonym(s) | CMV-creERT; CAG-CreERT2; CAGG-CreERT2; CAGGCre-ERTM; CAGGCre-ERtm line 5.8; CAGGS-CreErT; Cre-ER; Cre-ERTM; ER-cre; Tg(CAG-cre/Esr1)5Amc; Tg(cre/Esr1)5Amc; Tg(CAG-cre/Esr1)5Amc; Tg(cre/Esr1)5Amc; CAGGS-CreER; CAGGCre-ER |
| Promoter | ACTB, actin, beta, chicken |
| Expressed Gene | cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene, |
| Site of Expression | tamoxifen-inducible cre; widespread pattern of expression; tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice |
| Strain of Origin | (C57BL/6 x CBA)F1 |
| Chromosome | UN |
| General Note | Homozygous transgenic mice are not viable or fertile. Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In transgenic mice the mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 fusion protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing loxP sites flanking a sequence of interest, tamoxifen induced, Cre-mediated targeted deletions are generated in the offspring. Tamoxifen administration will induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. |
| Molecular Note | This transgene expresses a fusion protein consisting of Cre recombinase joined to the ligand-binding domain of a mouse estrogen receptor modified to bind to 4-hydroxytamoxifen, but not to endogenous estrogen. The CAG promoter, containing a chicken beta actin promoter/enhancer coupled with the cytomegalovirus immediate-early (CMV-IE) enhancer, drives high levels of expression in most tissues. In the presence of tamoxifen, the fusion protein is transported into the nucleus, where cre can excise loxP-flanked segments from conditionally modified genes. |
| Mutations Made By | Shigemi Hayashi, Columbia University |
| Allele Name | targeted mutation 2, Kelvin Y Kwan |
|---|---|
| Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
| Allele Synonym(s) | Trpa1 CKO |
| Gene Symbol and Name | Trpa1, transient receptor potential cation channel, subfamily A, member 1 |
| Gene Synonym(s) | ANKTM1; FEPS; Anktm1; FEPS1 |
| Strain of Origin | 129S6/SvEvTac |
| Chromosome | 1 |
| Molecular Note | LoxP sites were placed on either side of exons 22-24 (encoding the S5/S6 transmembrane domains) and an FRT-flanked neomycin resistance cassette inserted in the opposite transcriptional orientation in intron 24. The neomycin cassette was excised by crossing these mice to a Gt(ROSA)26Sor-driven Flp strain on a 129S4/SvJaeSor. |
| Mutations Made By | Kelvin Kwan, Rutgers University |
When maintained as a live colony, animals homozygous for the targeted mutation and hemizygous for the transgene may be intercrossed. Animals homozygous for the transgene are not believed to be viable.
Facility Barrier Level Descriptions
| Service | Genotype | Price |
|---|---|---|
| Please inquire |
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
| Frozen Mouse Embryo | $2,595.00 per straw or vial |
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