Overview

Also Known As: Trpa1 CKO/CKO deltaNeo Tg(cre/Esr1)5Amc/J

These compound mutant mice carry a floxed allele of the Trpa1 (transient receptor potential cation channel, subfamily A, member 1) gene in addition to a tamoxifen inducible cre transgene driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer (see Stock No. 004682). Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces cre recombination within the Trpa1 allele, excising the S5/S6 transmembrane domains. Animals not treated with tamoxifen display no observable phenotype.

Donating Investigator

David P. Corey, Harvard Medical School/HHMI

Genetic overview

Genetic Background	Generation

Tg(CAG-cre/Esr1*)5Amc

Allele Type
Transgenic (Recombinase-expressing, Inducible)

Trpa1^tm2Kykw

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Trpa1	transient receptor potential cation channel, subfamily A, member 1

View Genetics

Research Applications

Research Tools
Neurobiology Research

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

These compound mutant mice carry a floxed allele of the Trpa1 (transient receptor potential cation channel, subfamily A, member 1) gene in addition to a tamoxifen inducible cre transgene driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer (see Stock No. 004682).

Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces cre recombination within the Trpa1 allele, excising the S5/S6 transmembrane domains. Animals not treated with tamoxifen display no observable phenotype.

Development

This compound mutant strain was created by intercrossing a Trpa1 floxed strain with a tamoxifen-inducible cre mutation strain (see Stock No. 004682).

To create the Trpa1 floxed allele, loxP sites were placed on either side of the exons that encode the S5/S6 transmembrane domains. An FRT-flanked self-excising neomycin resistance cassette was initially placed within the floxed allele, and was deleted. The mutation was created with 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells.

This compound mutant strain is on a mixed genetic background that involves C57BL/6, CBA, and 129.

Expression Data

Expressed Gene	cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene,
Site of Expression	tamoxifen-inducible cre; widespread pattern of expression; tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice
Site of Expression

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Corey D; Kwan KY. 2011. Generation of a conditional allele of Trpa1 MGI Direct Data SubmissionMGI: J:169738

View All References

Genetics

Tg(CAG-cre/Esr1*)5Amc

Allele Symbol: Tg(CAG-cre/Esr1*)5Amc

Allele Name	transgene insertion 5, Andrew P McMahon
Allele Type	Transgenic (Recombinase-expressing, Inducible)
Allele Synonym(s)	transgene insertion 5, Andrew P McMahon; Tg(CAG-cre/Esr1*)5Amc
Gene Symbol and Name	Tg(CAG-cre/Esr1*)5Amc, transgene insertion 5, Andrew P McMahon
Gene Synonym(s)	CMV-creERT; CAG-CreERT2; CAGG-CreERT2; CAGGCre-ER^TM; CAGGCre-ERtm line 5.8; CAGGS-CreErT; Cre-ER; Cre-ERTM; ER-cre; Tg(CAG-cre/Esr1)5Amc; Tg(cre/Esr1)5Amc; Tg(CAG-cre/Esr1)5Amc; Tg(cre/Esr1)5Amc; CAGGS-CreER; CAGGCre-ER
Promoter	ACTB, actin, beta, chicken
Expressed Gene	cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene,
Site of Expression	tamoxifen-inducible cre; widespread pattern of expression; tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice
Strain of Origin	(C57BL/6 x CBA)F1
Chromosome	UN
General Note	Homozygous transgenic mice are not viable or fertile. Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In transgenic mice the mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 fusion protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing loxP sites flanking a sequence of interest, tamoxifen induced, Cre-mediated targeted deletions are generated in the offspring. Tamoxifen administration will induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice.
Molecular Note	This transgene expresses a fusion protein consisting of Cre recombinase joined to the ligand-binding domain of a mouse estrogen receptor modified to bind to 4-hydroxytamoxifen, but not to endogenous estrogen. The CAG promoter, containing a chicken beta actin promoter/enhancer coupled with the cytomegalovirus immediate-early (CMV-IE) enhancer, drives high levels of expression in most tissues. In the presence of tamoxifen, the fusion protein is transported into the nucleus, where cre can excise loxP-flanked segments from conditionally modified genes.
Mutations Made By	Shigemi Hayashi, Columbia University

Trpa1^tm2Kykw

Allele Symbol: Trpa1^tm2Kykw

Allele Name	targeted mutation 2, Kelvin Y Kwan
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	targeted mutation 2, Kelvin Y Kwan; Trpa1^tm2Kykw
Gene Symbol and Name	Trpa1, transient receptor potential cation channel, subfamily A, member 1
Gene Synonym(s)	ANKTM1; FEPS; Anktm1; FEPS1
Strain of Origin	129S6/SvEvTac
Chromosome	1
Molecular Note	LoxP sites were placed on either side of exons 22-24 (encoding the S5/S6 transmembrane domains) and an FRT-flanked neomycin resistance cassette inserted in the opposite transcriptional orientation in intron 24. The neomycin cassette was excised by crossing these mice to a Gt(ROSA)26Sor-driven Flp strain on a 129S4/SvJaeSor.
Mutations Made By	Kelvin Kwan, Rutgers University

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Inducible
- Genetics Research
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers: dorsal CNS marker
  - Tissue/Cell Markers
- Sensorineural Research
Neurobiology Research
- Behavioral and Learning Defects

Mammalian Phenotype Terms by Genotype

References

Corey D; Kwan KY. 2011. Generation of a conditional allele of Trpa1 MGI Direct Data SubmissionMGI: J:169738

Additional - Trpa1^tm2Kykw related

Additional - Tg(CAG-cre/Esr1*)5Amc related

Technical Support

Contact Technical Support

Genotyping Protocols
- High Resolution Melting: Tg(CAG-cre)
- High Resolution Melting: Trpa1^tm1kykw
- Standard PCR: Trpa1^tm2Kykw
- Genotyping resources and troubleshooting
Breeding Considerations

When maintained as a live colony, animals homozygous for the targeted mutation and hemizygous for the transgene may be intercrossed. Animals homozygous for the transgene are not believed to be viable.
- Additional Breeding and Husbandry Support
Mating System
- See "Breeding Considerations"
Citation
When using the STOCK Trpa1^tm2Kykw Tg(CAG-cre/Esr1*)5Amc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008813 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous forTrpa1<tm2Kykw> and Hemizygous or non carrier for Tg(CAG-cre/Esr1*)5Amc

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: Trpa1 CKO/CKO deltaNeo Tg(cre/Esr1)5Amc/J

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(CAG-cre/Esr1*)5Amc

Allele Symbol: Tg(CAG-cre/Esr1*)5Amc

Trpa1tm2Kykw

Allele Symbol: Trpa1tm2Kykw

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Trpa1tm2Kykw related

Additional - Tg(CAG-cre/Esr1*)5Amc related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Trpa1^tm2Kykw

Allele Symbol: Trpa1^tm2Kykw

Additional - Trpa1^tm2Kykw related