Mice hemizygous for the mouse Lactb (lactamase, beta) transgene have a higher fat-mass-to-lean-mass (FMLM) ratio compared to wild-type littermates when fed a 6% fat diet. Over time the FMLM ratio difference between transgenic and wild-type animals increases. This mutant mouse strain may be useful in studies of metabolic syndrome and obesity.
Aldons J. Lusis, University of California at Los Angeles
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice hemizygous for the mouse Lactb (lactamase, beta) transgene are viable, fertile, normal in size and do not display any gross behavioral abnormalities. The transgene encompasses the entire mouse structural gene and its regulatory sequences. Transgenic mice have a higher fat-mass-to-lean-mass (FMLM) ratio compared to wild-type littermates when fed a 6% fat diet. Over time the FMLM ratio difference between transgenic and wild-type animals increases. This mutant mouse strain may be useful in studies of metabolic syndrome and obesity.
The murine bacterial artificial chromosome (BAC) RP23-3H18 containing the entire Lactb gene with minimal overlaps of neighboring genes was microinjected into the pronucleus of FVB/N fertilized eggs. Founder line 74.2 was subsequently established. The Donating Investigator maintained the strain by crossing hemizygotes to wildtype mice and has not attempted to make the strain homozygous.
Expressed Gene | Lactb, lactamase, beta, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 74.2, Aldons J Lusis |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Lactb)74.2Lus, transgene insertion 74.2, Aldons J Lusis |
Gene Synonym(s) | |
Promoter | Lactb, lactamase, beta, mouse, laboratory |
Expressed Gene | Lactb, lactamase, beta, mouse, laboratory |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The murine bacterial artificial chromosome (BAC) RP23-3H18 containing the entire Lactb gene with minimal overlaps of neighboring genes was microinjected into the pronucleus of FVB/N fertilized eggs. Founder line 74.2 was subsequently established. |
Mutations Made By | Josh Deignan, UCLA |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator maintained the strain by crossing hemizygotes to wildtype mice and has not attempted to make the strain homozygous.
When using the FVB-Tg(Lactb)74.2Lus/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008784 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non-carrier for Tg(Lactb)74.2Lus |
Frozen Mouse Embryo | FVB-Tg(Lactb)74.2Lus/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Lactb)74.2Lus/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Lactb)74.2Lus/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Lactb)74.2Lus/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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