In homozygotes from this Zbtb7b (zinc finger and BTB domain-containing 7B; Th-POK) targeted mutation strain, CD4+ T cells are almost completely absent because of a redirection of MHC class II restricted T cells to the cytotoxic lineage.
Dr. Dan R. Littman, New York University Medical Center
In homozygotes from this BTB/POZ domain-containing zinc finger transcription factor (Th-POK) targeted mutation strain, CD4+ T cells are almost completely absent because of a redirection of MHC class II restricted T cells to the cytotoxic lineage.
LoxP sites were placed on either side of the exon2-3 region and a loxP-PGK-neomycin resistance cassette was introduced 1.1 kb upstream of the initiation codon in the sense orientation. The mutation was created using B6(Cg)-Tyrc-2J/J-derived embryonic stem (ES) cells.
The PGK-neomycin cassette, exon2 and exon 3 were deleted in vivo by crossing the mice to EIIA-Cre mice to leave a single loxP site remains.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Allele Name||targeted mutation 1.2, Dan R Littman|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Zbtb7b, zinc finger and BTB domain containing 7B|
|Strain of Origin||B6(Cg)-Tyrc-2J/J|
|Molecular Note||Mice carrying the Zbtb7btm1Litt allele were crossed with a cre transgenic strain to remove the entire coding sequence of the gene locus from germline cells, leaving behind a single loxP site. No protein expression was detected in immunoblot analysis of thymocytes.|
|Mutations Made By|| |
Dr. Dan Littman, New York University Medical Center
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the C57BL/6-Zbtb7btm1.2Litt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008775 in your Materials and Methods section.