B6(Cg)-Rorc^tm3Litt/J

Stock number: 008771
Product name: Rorγt^fl
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking the exon 3-6 region of the Rorc gene. This strain is useful in eliminating tissue-specific expression of orphan nuclear receptor RORγ, which results in abnormality of lymphoid organ development, survival of CD4⁺ CD8⁺ double positive thymocytes, and differentiation of Th17 helper T cells.

Detailed description

These mice possess loxP sites on either side of the exon 3-6 region of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of orphan nuclear receptor RORgamma, including the RORgammaT thymus-specific isoform. Germline deletion of RORgamma and RORgammaT induces an abnormality in lymphoid organ development, survival of CD4⁺ CD8⁺ double positive thymocytes, and differentiation of Th17 helper T cells.

Development

Exons 3-6 of the targeted gene were flanked by loxP sites. A floxed neomycin resistance gene used in the construction of this strain was deleted by partial recombination. C57BL/6-derived CY2.4 embyronic stem (ES) cells were used to create the mutation. The strain was maintained on a C57BL/6 background (see SNP note below) by the donating laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Rorc^tm3Litt
Name: targeted mutation 3, Dan R Littman
MGI accession ID: MGI:3818459
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Rorc
Marker name: RAR-related orphan receptor gamma
Chromosome: 3
Strain of origin: B6(Cg)-Tyr^c-2J
Molecular note: Exons 3-6 of the targeted gene are flanked by loxP sites. A floxed neomycin resistance gene used in the construction of this strain was deleted by partial recombination.

Allele

MGI accession ID: MGI:104856
Marker symbol: Rorc
Marker name: RAR-related orphan receptor gamma
Chromosome: 3