These floxed mutant mice possess loxP sites flanking the exon 3-6 region of the Rorc gene. This strain is useful in eliminating tissue-specific expression of orphan nuclear receptor RORγ, which results in abnormality of lymphoid organ development, survival of CD4+ CD8+ double positive thymocytes, and differentiation of Th17 helper T cells.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
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F?+N1F11
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Rorc | RAR-related orphan receptor gamma |
Marker Symbol | Marker Name | |
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Rorc | RAR-related orphan receptor gamma |
These mice possess loxP sites on either side of the exon 3-6 region of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of orphan nuclear receptor RORgamma, including the RORgammaT thymus-specific isoform. Germline deletion of RORgamma and RORgammaT induces an abnormality in lymphoid organ development, survival of CD4+ CD8+ double positive thymocytes, and differentiation of Th17 helper T cells.
Exons 3-6 of the targeted gene were flanked by loxP sites. A floxed neomycin resistance gene used in the construction of this strain was deleted by partial recombination. C57BL/6-derived CY2.4 embyronic stem (ES) cells were used to create the mutation. The strain was maintained on a C57BL/6 background (see SNP note below) by the donating laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 3, Dan R Littman |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | RORgammaflox |
Gene Symbol and Name | Rorc, RAR-related orphan receptor gamma |
Gene Synonym(s) | |
Strain of Origin | B6(Cg)-Tyrc-2J |
Chromosome | 3 |
Molecular Note | Exons 3-6 of the targeted gene are flanked by loxP sites. A floxed neomycin resistance gene used in the construction of this strain was deleted by partial recombination. |
Marker Synonym(s) | |
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Chromosome(s) | 3 |
When using the Rorγtfl mouse strain in a publication, please cite the originating article(s) and include JAX stock #008771 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Rorc<tm3Litt> |
Frozen Mouse Embryo | B6(Cg)-Rorc<tm3Litt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Rorc<tm3Litt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Rorc<tm3Litt>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Rorc<tm3Litt>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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