These mice possess loxP sites on either side of exon 5 in the targeted gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.
When bred to CD4-cre mice, homozyogous animals have reduced peripheral T cell numbers, abnormal CD4/CD8 expression, and altered response to antigen receptor stimulation. In addition, these mice show autoimmune colitis and asthma-like syndrome.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Cbfb | core binding factor beta |
These mice possess loxP sites on either side of exon 5 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.
When bred to CD4-cre mice, homozyogous animals have reduced peripheral T cell numbers, abnormal CD4/CD8 expression, and altered response to antigen receptor stimulation. In addition, these mice show autoimmune colitis and asthma-like syndrome.
A floxed neomycin resistance cassette was placed in intron 4 and a loxP site was placed in intron 5. The mutation was introduced using 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Cre expression in the embryonic stem cells excised the floxed neomycin cassette, leaving exon 5 flanked by loxP sites. This line was backcrossed to C57BL/6 for eight generations by the donating laboratory.
Allele Name | targeted mutation 1, Ichiro Taniuchi |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Cbfbetaf; Cbfbetaflox |
Gene Symbol and Name | Cbfb, core binding factor beta |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 8 |
Molecular Note | A floxed neomycin resistance cassette was placed adjacent to exon 5 and a loxP site was placed in the other flanking intron. The neomycin resistance cassette was subsequently removed by transient expression of Cre recombinase, leaving exon 5 flanked by loxP sites |
Mutations Made By | Ichiro Taniuchi, Inst. of Physical and Chemical Research |
When maintained as a live colony, heterozygous or homozygous mice may be bred.
When using the B6.129P2-Cbfbtm1Itan/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008765 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Cbfb<tm1Itan> |
Frozen Mouse Embryo | B6.129P2-Cbfb<tm1Itan>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Cbfb<tm1Itan>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Cbfb<tm1Itan>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Cbfb<tm1Itan>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.