B6.129P2-Cbfb^tm1Itan/J

Stock number: 008765
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These mice possess loxP sites on either side of exon 5 in the targeted gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When bred to CD4-cre mice, homozyogous animals have reduced peripheral T cell numbers, abnormal CD4/CD8 expression, and altered response to antigen receptor stimulation. In addition, these mice show autoimmune colitis and asthma-like syndrome.

Detailed description

These mice possess loxP sites on either side of exon 5 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When bred to CD4-cre mice, homozyogous animals have reduced peripheral T cell numbers, abnormal CD4/CD8 expression, and altered response to antigen receptor stimulation. In addition, these mice show autoimmune colitis and asthma-like syndrome.

Development

A floxed neomycin resistance cassette was placed in intron 4 and a loxP site was placed in intron 5. The mutation was introduced using 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Cre expression in the embryonic stem cells excised the floxed neomycin cassette, leaving exon 5 flanked by loxP sites. This line was backcrossed to C57BL/6 for eight generations by the donating laboratory.

Allele

Symbol: Cbfb^tm1Itan
Name: targeted mutation 1, Ichiro Taniuchi
MGI accession ID: MGI:3761812
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Cbfb
Marker name: core binding factor beta
Chromosome: 8
Strain of origin: 129P2/OlaHsd
Molecular note: A floxed neomycin resistance cassette was placed adjacent to exon 5 and a loxP site was placed in the other flanking intron. The neomycin resistance cassette was subsequently removed by transient expression of Cre recombinase, leaving exon 5 flanked by loxP sites