Mice harboring both the Ins2-rtTA (or RIP7-rtTA) transgene and the tet-DTA (or tetO-DTA) transgene have doxycycline-inducible expression of DTA in pancreatic beta cells; i.e. addition of doxycycline results in ablation of pancreatic beta cells.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inducible, Inserted expressed sequence) |
Allele Type |
---|
Transgenic (Transactivator) |
This strain was generated by breeding Stock No. 008168 and Stock No. 008250 together at The Jackson Laboratory. The resulting double transgenic colony was established as Stock No. 008755.
The Ins2-rtTA (or RIP7-rtTA) transgene expresses the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 (Ins2) promoter. The tet-DTA (or tetO-DTA) transgene expresses diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) and a cytomegalovirus minimal promoter. Mice harboring both of these transgenes has doxycycline-inducible expression of DTA in pancreatic beta cells; i.e. addition of the tetracycline analogue doxycycline (dox) results in ablation of pancreatic beta cells.
This strain was generated by breeding Stock No. 008168 and Stock No. 008250 together at The Jackson Laboratory. The resulting double transgenic colony was established (as Stock No. 008755). This strain was subsequently maintained by breeding mice carrying both the RIP7-rtTA transgene and tet-DTA transgene together. The genetic background is a mix of C57BL/6, CBA, and outbred ICR.
Expressed Gene | Dta, Diphtheria toxin A chain, |
---|---|
Site of Expression | pancreatic beta cells |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | pancreatic beta cells |
Allele Name | transgene insertion 1, Glenn I Fishman |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | tet-DTA; tetODTA/+; tetO-DTA |
Gene Symbol and Name | Tg(tetO-DTA)1Gfi, transgene insertion 1, Glenn I Fishman |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Dta, Diphtheria toxin A chain, |
Site of Expression | pancreatic beta cells |
Strain of Origin | (C57BL/6 x CBA)F2 |
Chromosome | UN |
General Note | Phenotypic Similarity to Human Syndrome: Narcolepsy J:211045, J:211046 |
Molecular Note | A transgenic construct was created, containing diphtheria toxin A sequence (DTA) under the control of heptamerized tetracycline operator, tetO sequences fused to a cytomegalovirus minimal promoter. |
Allele Name | transgene insertion 2, Shimon Efrat |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | Ins-rtTA; RIP7-rtTA; RiprtTA |
Gene Symbol and Name | Tg(Ins2-rtTA)2Efr, transgene insertion 2, Shimon Efrat |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | pancreatic beta cells |
Strain of Origin | (C57BL/6 x CBA)F2 |
Chromosome | UN |
Molecular Note | A transgenic construct containing 9.5kb 5' regulatory sequence and first intron of the rat insulin 2 gene (RIP7) controlling the reverse tetracycline transactivator (rtTA), as well as the polyadenylation site sequence of the RIP7 gene was injected into fertilized (C57BL/6 X CBA)F2 mouse eggs. Founder line 2 with 10 copies of the transgene was established. When mice are crossed to transgenic mice carrying reporter genes, transgene expression is detected only in pancreatic islet beta cells. |
This strain was generated by breeding Stock No. 008168 and Stock No. 008250 together at The Jackson Laboratory. The resulting double transgenic colony was established (as Stock No. 008755). This strain was subsequently maintained by breeding mice carrying both the RIP7-rtTA transgene and tet-DTA transgene together. The genetic background is a mix of C57BL/6, CBA, and outbred ICR.
When using the STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008755 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Ins2-rtTA)2Efr and Tg(teto-DTA)1Gfi |
Frozen Mouse Embryo | STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J Frozen Embryos | $3373.50 |
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