STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J

Stock number: 008755
Research areas: Diabetes and Obesity Research, Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Metabolism Research, Neurobiology Research, Research Tools

Description

Mice harboring both the Ins2-rtTA (or RIP7-rtTA) transgene and the tet-DTA (or tetO-DTA) transgene have doxycycline-inducible expression of DTA in pancreatic beta cells; i.e. addition of doxycycline results in ablation of pancreatic beta cells.

Detailed description

This strain was generated by breeding Stock No. 008168 and Stock No. 008250 together at The Jackson Laboratory. The resulting double transgenic colony was established as Stock No. 008755.

The Ins2-rtTA (or RIP7-rtTA) transgene expresses the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 (Ins2) promoter. The tet-DTA (or tetO-DTA) transgene expresses diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) and a cytomegalovirus minimal promoter. Mice harboring both of these transgenes has doxycycline-inducible expression of DTA in pancreatic beta cells; i.e. addition of the tetracycline analogue doxycycline (dox) results in ablation of pancreatic beta cells.

Development

This strain was generated by breeding Stock No. 008168 and Stock No. 008250 together at The Jackson Laboratory. The resulting double transgenic colony was established (as Stock No. 008755). This strain was subsequently maintained by breeding mice carrying both the RIP7-rtTA transgene and tet-DTA transgene together. The genetic background is a mix of C57BL/6, CBA, and outbred ICR.

Allele

Symbol: Tg(tetO-DTA)1Gfi
Name: transgene insertion 1, Glenn I Fishman
MGI accession ID: MGI:3767624
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Synonyms: tet-DTA; tetO^DTA/+; tetO-DTA
Marker symbol: Tg(tetO-DTA)1Gfi
Marker name: transgene insertion 1, Glenn I Fishman
Marker synonyms: tet-DTA; tetO^DTA/+
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F2
Expressed genes: Dta,Diphtheria toxin A chain,
Site of expression: Tet-inducible/controllable expression of diphtheria toxin A
Molecular note: A transgenic construct was created, containing diphtheria toxin A sequence (DTA) under the control of heptamerized tetracycline operator, tetO sequences fused to a cytomegalovirus minimal promoter.
General note: Phenotypic Similarity to Human Syndrome: Narcolepsy J:211045, J:211046

Allele

Symbol: Tg(Ins2-rtTA)2Efr
Name: transgene insertion 2, Shimon Efrat
MGI accession ID: MGI:3770667
Generation method: Transgenic
Attributes: Transactivator
Synonyms: Ins-rtTA; RIP7-rtTA; RiprtTA
Marker symbol: Tg(Ins2-rtTA)2Efr
Marker name: transgene insertion 2, Shimon Efrat
Marker synonyms: Ins-rtTA; RIP7-rtTA; RiprtTA
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F2
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: pancreatic beta cells
Molecular note: A transgenic construct containing 9.5kb 5' regulatory sequence and first intron of the rat insulin 2 gene (RIP7) controlling the reverse tetracycline transactivator (rtTA), as well as the polyadenylation site sequence of the RIP7 gene was injected into fertilized (C57BL/6 X CBA)F2 mouse eggs. Founder line 2 with 10 copies of the transgene was established. When mice are crossed to transgenic mice carrying reporter genes, transgene expression is detected only in pancreatic islet beta cells.